P5333

Pleiotrophin human

≥97% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder, suitable for cell culture

• Synonym(s): HARP, HB-GAM, Heparin affinity regulatory peptide, Heparin-binding growth factor, Heparin-binding growth-associated molecule, PTN
• MDL number: MFCD01324256
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in baculovirus infected Sf21 cells
• Assay: ≥97% (SDS-PAGE)
• form: lyophilized powder
• mol wt: predicted mol wt 15.3 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P21246
• storage temp.: −20°C
• Gene Information: human ... PTN(5764)

Description

General description

Pleiotrophin (PTN) is a multifunctional growth factor. The protein coded by the PTN gene is a member of the heparin-binding cytokines family. This gene is located on human chromosome 7. Pleiotrophin is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding growth-associated molecule (HB-GAM) and osteoblast-specific factor (OSF-1). This gene is highly expressed in brain, uterus, gut, muscle, lung and skin. Pleiotrophin mRNA is expressed in osteoblasts, chondrocytes, tumor cells, fibroblasts, astrocytes and Schwann cells.

Application

PTN is active in growth and development, and has mitogenic and neurite outgrowth activity. The gene for pleiotrophin is highly expressed in brain, uterus, gut, muscle, lung, and skin tissues

Biochem/physiol Actions

Pleiotrophin participates in growth and development. Pleiotrophin is mitogenic and displays neurite outgrowth activity. It stimulates tumor angiogenesis. There is extraordinary conservation between the amino acid sequences of bovine, human, and rat pleiotrophin.

Pleiotrophin may be the first member of a family of developmentally regulated cytokines. It is active in growth and development and the pleiotrophin gene is highly expressed in brain, uterus, gut, muscle, lung, and skin. Pleiotrophin mRNA is expressed in osteoblasts, chondrocytes, fibroblasts, astrocytes, Schwann cells, and tumor cells. Pleiotrophin is mitogenic and displays neurite outgrowth activity. It stimulates tumor angiogenesis. Pleiotrophin is also known as heparin-binding brain mitogen (HBBM), heparin-binding growth factor-8 (HBGF-8), heparin-binding growth-associated molecule (HB-GAM) and osteoblast-specific factor (OSF-1). There is extraordinary conservation between the amino acid sequences of bovine, human, and rat pleiotrophin.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The activity is measured in culture by the ability to enhance neurite outgrowth of cerebral cortical neurons from rat embryos.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves