The HT200 kit is approved for IVD use and is specifically approved for use with standard paraffin-embedded tissue cut at 4 or 5 microns. No procedures have been developed for possible use in 3D tissue structures for the quantification of Neuromelanin. It would be required to adapt the procedure for any use not outlined in the Instructions for Use. The time spent in the ammoniacal silver stain solution would need to be adjusted, and it is suggested to start at 15 minutes and proceed in increments of 15 minutes until acceptable staining is obtained for staining 3D tissue. If results are promising, it is recommended to possibly proceed in increments of 5 minutes to optimize the staining results. It is possible that the staining times for the other steps may also need to be adjusted.
HT200
Fontana-Masson Kit
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About This Item
shelf life
Expiry date on the label
Quality Segment
IVD
for in vitro diagnostic use
dilution
(for histology)
application(s)
hematology
histology
shipped in
wet ice
storage temp.
2-8°C
General description
Biochem/physiol Actions
1 of 1
This Item | |||
|---|---|---|---|
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 500 |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD for in vitro diagnostic use |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shelf life Expiry date on the label | shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. |
signalword
Danger
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Ox. Liq. 2 - Repr. 1B - Skin Corr. 1A
Storage Class
5.1B - Oxidizing hazardous materials
flash_point_f
Not applicable
flash_point_c
Not applicable
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Is there a procedure or information available for the use of the Fontana Masson staining kit in 3D tissue structures for the quantification of Neuromelanin?
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Is it possible to collect viable DNA from tissue stained with the Fontanna Masson HT200 kit?
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Collecting DNA and RNA from FFPE fixed tissues is a common practice, typically involving tissue from paraffin-embedded blocks or slides stained with Hematoxylin and Eosin. Collecting DNA and RNA from FFPE fixed tissues is a common practice, typically involving tissue from paraffin-embedded blocks or slides stained with Hematoxylin and Eosin.
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