GE29-0005-94
His GraviTrap™ TALON®
Cytiva 29-0005-94, pack of 10 columns
Synonym(s):
His GraviTrap
About This Item
Recommended Products
ligand
TALON® Superflow Cobalt-IMAC
packaging
pack of 10 columns
manufacturer/tradename
Cytiva 29-0005-94
bed volume
1 mL
column I.D.
15 mm
matrix
6% cross-linked agarose
particle size
60-160 μm
cleaning
2-14(Co2+-stripped medium)
working range
3-12(Co2+-stripped medium)
capacity
≤15 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)
storage temp.
2-8°C
General description
Application
Large sample volumes can be applied in one go, and the histidine-tagged Protein is effectively eluted in a small volume. LabMate PD-10 Buffer Reservoir can be connected to the columns for handling of sample volumes above 10 ml. Leakage of cobalt ions from Talon® Superflow medium is negligible.
Features and Benefits
- Convenient histidine-tagged purification with high purity (>90%) and binding capacity of up to 15 mg/column.
- Ready-to-use gravity flow column prepacked with 1 mL Talon® Superflow cobalt-IMAC (immobilized metal affinity chromatography) medium offering a different selectivity compared to nickel-charged media.
- One run takes about 30 minutes, and large sample volumes can be applied all at once.
Storage and Stability
Analysis Note
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
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Articles
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from Cytiva.
Protocols
This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.
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