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Merck

FLAAB

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer

lyophilized powder

Synonym(s):

ATP Assay Dilution Buffer, Assay Mix Buffer, Assay Mix for ATP

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About This Item

NACRES:
NA.84
eCl@ss:
32160414
UNSPSC Code:
41116133
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form

lyophilized powder

Quality Level

technique(s)

activity assay: suitable

storage temp.

−20°C

General description

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer is a component of the ATP bioluminescent assay kit that may be employed for the quantitative bioluminescent determination of ATP in experimental samples.

Application

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer has been used to determine the level of adenosine kinase activity in isolated sections of mouse hippocampi. It has also been used to measure the ATP content in oocytes and harderian gland(HG) tissue homogenates.

Preparation Note

Adenosine 5′-triphosphate (ATP) assay mix dilution buffer contains MgSO4, dithiothreitol (DTT), ethylene diamine tetraacetic acid (EDTA, bovine serum albumin (BSA), and tricine buffer salts.
Reconstitute with 50 mL of sterile water.


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Nicolette Gouder et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 24(3), 692-701 (2004-01-23)
Endogenous adenosine in the brain is thought to prevent the development and spread of seizures via a tonic anticonvulsant effect. Brain levels of adenosine are primarily regulated by the activity of adenosine kinase. To establish a link between adenosine kinase
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A wealth of microbial eukaryotes is adapted to life in oxygen-deficient marine environments. Evidence is accumulating that some of these eukaryotes survive anoxia by employing dissimilatory nitrate reduction, a strategy that otherwise is widespread in prokaryotes. Here, we report on
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Anti-tumor immune responses have been linked to the regulated release of ATP from apoptotic cancer cells to engage P2 purinergic receptor signaling cascades in nearby leukocytes. We used the Jurkat T cell acute lymphocytic leukemia model to characterize the role



Global Trade Item Number

SKUGTIN
FLAAB-10VL04061833296325
FLAAB-5VL04061838163530
FLAAB-1VL04061838172716