The molecular weight of ExtrAvidin® is 73 kDa.
E2511
ExtrAvidin®
essentially salt-free, lyophilized powder
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About This Item
Form:
essentially salt-free, lyophilized powder
Pricing and availability is not currently available.
form
essentially salt-free, lyophilized powder
Quality Segment
technique(s)
dot blot: suitable, immunohistochemistry: suitable, indirect ELISA: suitable, radioimmunoassay: suitable
shipped in
wet ice
storage temp.
2-8°C
Application
ExtrAvidin® has been used to tetramerize aliquots to generate HLA-A2 tetramers.
Biochem/physiol Actions
Binding Activity: at least 10 μg biotin per mg
ExtrAvidin® is prepared from egg white avidin. It is a tetrameric protein containing four high affinity binding sites for biotin. It combines the high specific activity of avidin with the low background staining of streptavidin, a biotin binding protein produced by the bacteria Streptomyces avidinii. It binds biotin with the high affinity of egg white avidin, however, it does not exhibit the unwanted non-specific binding reported for egg white avidin at physiological pH, such as the staining of mast cells.
Other Notes
A modified form of affinity purified egg white avidin.
Legal Information
ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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| description essentially salt-free, lyophilized powder | description buffered aqueous solution | description buffered aqueous solution | description buffered aqueous solution |
| technique(s) indirect ELISA: suitable, radioimmunoassay: suitable, dot blot: suitable, immunohistochemistry: suitable | technique(s) direct ELISA: 1:2,000, dot blot: 1:4,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 | technique(s) dot blot: 1:300,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using human tissues sections, indirect ELISA: 1:60,000 | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 20 μg/mL using human tissues, indirect immunofluorescence: 1:200 |
| shipped in wet ice | shipped in wet ice | shipped in ambient | shipped in dry ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form essentially salt-free, lyophilized powder | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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