MDCK-SIAT1 Cell Line canine
5071502, canine kidney, Epithelial
Synonym(s):
MDCK-SIAT1 Cells
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About This Item
UNSPSC Code:
41106514
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product name
MDCK-SIAT1 Cell Line canine, 05071502
biological source
canine kidney
description
Canine Cocker Spaniel Kidney Sialic Acid Over Expression
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Enhanced expression of 6-linked sialic acids
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Canine Cocker Spaniel Kidney Sialic Acid Over Expression
Cell Line Description
This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.
Culture Medium
DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 37°C; 5% CO2.
Other Notes
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Certificates of Analysis (COA)
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