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C3020

Sigma-Aldrich

Chitin azure

chitinase substrate

Synonym(s):

Colloid chitin azure

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32

form

powder

General description

Chitin azure is sensitive, feasible and reproducible low cost substrate, which is used for the assay of chitinase activity.

Application

Chitin azure has been used as a substrate to determine chitinase activity.

Other Notes

Chitin from crab shells covalently linked with Remazol Brilliant Violet 5R (RBV) dye.

Substrates

A substrate for chitinase

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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NEW SUBSTRATES FOR USE WITH CHITINASES.
R H HACKMAN et al.
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Vibrio carchariae chitinase A (EC3.2.1.14) is a family-18 glycosyl hydrolase and comprises three distinct structural domains: i) the amino terminal chitin binding domain (ChBD); ii) the (alpha/beta)8 TIM barrel catalytic domain (CatD); and iii) the alpha + beta insertion domain.
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A large percentage of crop loss is due to insect damage, especially caterpillar damage. Plant chitinases are considered excellent candidates to combat these insects since they can degrade chitin in peritrophic matrix (PM), an important protective structure in caterpillar midgut.

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