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A2231

Sigma-Aldrich

Anti-Axin1 (C-terminal region) antibody produced in rabbit

~2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Axin, Anti-Axis Inhibitor 1, Anti-Axis inhibition protein 1

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~130 kDa

species reactivity

mouse, rat, human

concentration

~2.5 mg/mL

technique(s)

immunohistochemistry: suitable
western blot: 0.5-1 μg/mL using HEK-293 cells expressing mouse axin1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AXIN1(8312)
mouse ... Axin1(12005)
rat ... Axin1(79257)

General description

Axin1 contains two conserved domains, an N-terminal regulator of G-protein signaling (RGS) domain, and a C-terminal DIX domain. The C-terminal region of axin1 is important for homodimerization, whereas the central region binds β-catenin and GSK-3b.

Immunogen

synthetic peptide corresponding to amino acids 815-833 located near the C-terminal region of human axin1, conjugated to KLH. This sequence is identical in rat and mouse axin1 and highly conserved (83% identity) in human, rat, and mouse axin2.

Application

Immunohistochemistry was performed using rabbit anti-axin antibody to visualize Axin in the mouse developing cortex. Mouse brains had been previously harvested and fixed in 4% paraformaldhyde and cryoprotected in 20% sucrose. Axin was seen in the soma and axons of neurons.
Rabbit anti-axin1 (C-terminal region) antibody can be used at a concentration of 0.5-1μg/ml in western blot assays using HEK-293 cells expressing mouse axin1.

Biochem/physiol Actions

Axin1 (axis inhibitor-1 or axin) functions as a negative regulator of Wnt signaling. Axin1 is known to associate with several members of the Wnt pathway such as β-catenin, glycogen synthase kinase-3b (GSK-3b), dishevelled (Dsh) and adenomatous polyposis coli (APC).
Axin1 (axis inhibitor-1, axin) enhances the phosphorylation of β-catenin by Glycogen synthase kinase 3 β (GSK-3b). Axin1 is involved in the degradation of β-catenin by acting as a scaffold to form a complex between β-catenin, adenomatous polyposis coli (APC) and GSK-3b thus, facilitating β-catenin phosphorylation by GSK-3b. Axin1 itself is phosphorylated and its activity regulated by GSK-3b. Axin1 phosphorylation is reduced by Wnt signaling, leading to decreased affinity of interaction of axin1 and β-catenin. This results in the release of β-catenin from the degradation complex and increased stability of β-catenin in the cell. Axin1 is destabilized by Wnt signaling and its levels in the cell are reduced. Axin isoform axin2 (conductin, axil) is 44% identical to Axin1 and appears to play a similar role to Axin1 in the Wnt signaling pathway.

Physical form

Solution in 0.01 mM phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Axin phosphorylation in both Wnt-off and Wnt-on states requires the tumor suppressor APC
Tacchelly-Benites O, et al.
PLoS Genetics, 14(2), e1007178-e1007178 (2018)
Mouse axin and axin2/conductin proteins are functionally equivalent in vivo
Chia IV and Costantini F
Molecular and Cellular Biology, 25(11), 4371-4376 (2005)
New insights into the regulation of Axin function in canonical Wnt signaling pathway
Song X, et al.
Protein & cell, 5(3), 186-193 (2014)
F Fagotto et al.
The Journal of cell biology, 145(4), 741-756 (1999-05-20)
Axin was identified as a regulator of embryonic axis induction in vertebrates that inhibits the Wnt signal transduction pathway. Epistasis experiments in frog embryos indicated that Axin functioned downstream of glycogen synthase kinase 3beta (GSK3beta) and upstream of beta-catenin, and
L Zeng et al.
Cell, 90(1), 181-192 (1997-07-11)
Mutations at the mouse Fused locus have pleiotropic developmental effects, including the formation of axial duplications in homozygous embryos. The product of the Fused locus, Axin, displays similarities to RGS (Regulators of G-Protein Signaling) and Dishevelled proteins. Mutant Fused alleles

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