Some applications for use of 2´,7´-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC. See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2´,7´-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
35848
2′,7′-Dichlorofluorescein
BioReagent, suitable for fluorescence, ≥90% (T)
Synonym(s):
DCF, Dichlorofluorescein
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Empirical Formula (Hill Notation):
C20H10Cl2O5
CAS Number:
Molecular Weight:
401.20
Beilstein:
58009
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.32
Pricing and availability is not currently available.
Recommended Products
product line
BioReagent
Quality Level
Assay
≥90% (T)
form
solid
mp
280 °C (dec.) (lit.)
fluorescence
λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0
suitability
suitable for fluorescence
SMILES string
Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
InChI
1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H
InChI key
VFNKZQNIXUFLBC-UHFFFAOYSA-N
Looking for similar products? Visit Product Comparison Guide
General description
2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. [1] The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[2]
Application
2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. [3] It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
G Gübitz et al.
Journal of chromatography, 117(2), 337-343 (1976-02-18)
An in situ fluorimetric method has been developed for the quantitation of gluconic and lactobionic acids and their salts in tablet formulations. The method is based on glycol cleavage with lead tetraacetate followed by treatment with dichlorofluorescein. Calcium gluconate and
H. Watanabe et al.
Analytical Sciences, 2, 461-461 (1986)
W.D. Pfeffer et al.
Journal of Chromatography A, 506, 401-401 (1990)
Masahito Miura et al.
Journal of molecular and cellular cardiology, 56, 97-105 (2012-12-26)
Ca(2+) waves are initiated not only by Ca(2+) leak from the sarcoplasmic reticulum (SR), but also by Ca(2+) dissociation from the myofilaments in the myocardium with nonuniform contraction. We investigated whether contractile properties and the production of reactive oxygen species
Xiuping Chen et al.
Free radical research, 44(6), 587-604 (2010-04-08)
Reactive oxygen species (ROS) are critically important chemical intermediates in biological studies, due to their multiple physiologically essential functions and their often pathologically deleterious effects. Consequently, it is vital that their presence in biological samples has to be quantifiable. However
-
In what types of applications can 2´,7´-Dichlorofluorescein be used?
1 answer-
Helpful?
-
-
What excitation /emission wavelengths are best for fluorescence detection of 2´,7´-Dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
Helpful?
-
-
What can be used to make a solution of 2´,7´-dichlorofluorescein?
1 answer-
2´,7´-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2´,7´-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
