1. Phthaldialdehyde Reagent is provided in an amber glass bottle.
2. Work under a fume hood. Do not inhale substance/mixture. Avoid generation of vapours/aerosols. Store in a tightly closed. Keep it in a well-ventilated place.
3. To store under inert gas, open the cap and then direct a gentle stream of nitrogen or argon gas down into the vial for a few seconds. Closely tightly and store at t 2–8 °C.
P0532
Phthaldialdehyde Reagent
Solution Complete
Synonym(s):
OPA
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.21
Pricing and availability is not currently available.
Recommended Products
Quality Level
storage temp.
2-8°C
SMILES string
[H]C(=O)c1ccccc1C([H])=O
InChI
1S/C8H6O2/c9-5-7-3-1-2-4-8(7)6-10/h1-6H
InChI key
ZWLUXSQADUDCSB-UHFFFAOYSA-N
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Application
Precolumn derivatization reagent for primary amines and amino acids. The fluorescent derivative can be detected by reverse-phase HPLC. The reaction requires OPA, primary amine and a sulfhydryl. In the presence of excess sulfhydryl, amines can be quantitated. In the presence of excess amine, sulfhydryls can be quantitated.
Other Notes
Contains 1 mg o-phthaldialdehyde (P0657) per mL solution with 2-mercaptoethanol as the sulfhydryl moiety.
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Description
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1B - Skin Sens. 1
WGK
WGK 3
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1) What type of container this reagent stored at when you supply? 2) After I use for the first time, can you pls explain about proper storing? Should I purge inside the container with N2 gas? or just keep the bottle in N2 environment?
1 answer-
Helpful?
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Can you please explain about the stability of the reagent? When I open the bottle during derivatization time, should I maintain inert environment too?
1 answer-
Special care should be taken to ensure the product is maintained in an inert atmosphere (nitrogen or argon) at 2–8 °C since improper storage or handling conditions may render the solution ineffective. Please review the product information sheet for more details.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/300/p0532dat.pdfHelpful?
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이 시약이 HPLC 에 사용될 수 있나요 ?? 등급이 따로 기재되어 있지 않아서 문의드립니다.
1 answer-
While the grade of the Phthaldialdehyde Reagent is not listed, it is used for the pre-column derivatization of primary amines and amino acids for HPLC analysis. So this reagent can be used in HPLC analysis.
Helpful?
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I would like to ask if this is a readu-to-use reagent for amino acid derivatization purposes or if it needs further mixing with other reagents, e.g. mercaptoethanol and so on.
1 answer-
This product already contains mercaptoethanol and other reagents, as can be seen on the SDS. It is ready to use. Please see the product information sheet for more information around storage and use: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/326/300/p0532dat.pdf.
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