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Lipopolysaccharide

Name: Lipopolysaccharide
Brand: MM

from E. coli J5 mutant, Nitric oxide synthase stimulator, lyophilized

Synonym(s):

Lipopolysaccharide, E. coli J5

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About This Item

CAS Number:

93572-42-0

UNSPSC Code:

12352201

NACRES:

NA.77

Form:

lyophilized

Storage condition:

OK to freeze

Pricing and availability is not currently available.

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Properties

Product Name

Lipopolysaccharide, E. coli J5, Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Quality Segment

100

description

RTECS - OJ0895400

form

lyophilized

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

impurities

<2.5% Protein, 5.0-10.0% 2-keto-3-deoxyoctonate (KDO)

solubility

water: 1 mg/mL

anion traces

phosphate (PO₄^3-): 6.0-15.0%

shipped in

ambient

storage temp.

2-8°C

Description

General description

Mutant of E. coli 0111:B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes. Highly purified preparation.

Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Inducible nitric oxide synthase

Product does not compete with ATP.

Reversible: no

Preparation Note

Following reconstitution refrigerate (4°C). Stock solutions are stable for up to 1 year at 4°C.

LPS will form a suspension in H₂O; slight warming and vortexing may be required to achieve complete dispersion in H₂O. Addition of 0.5% triethylamine will also help with dispersion; triethylamine is very basic and can be neutralized with Trix-HCl to avoid hydrolysis of the fatty acid chains.

Other Notes

Hattori, S., et al. 1995. Biochem. Mol. Biol. Int.35, 177.
Mayeux, P.R., et al. 1995. Biochem. Pharmacol.49, 115.
Norimatsu, M., et al. 1995. Infect. Immun.63, 1122.
Feinstein, D.L., et al. 1993. J. Neurochem.60, 1945.
Zhang, Y.H., et al. 1993. Infect. Immun.61, 5044.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

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This Item	437627	437650	L2018
Sigma-Aldrich 437620 Lipopolysaccharide, E. coli J5	Sigma-Aldrich 437627 Lipopolysaccharide, E. coli O111: B4	Sigma-Aldrich 437650 Lipopolysaccharide, Salmonella typhimurium	Sigma-Aldrich L2018 Lipopolysaccharides from Escherichia coli K-235
description Mutant of E. coli O111: B4. Stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes.	description Lipopolysaccharide, E. coli O111: B4 stimulates the activity of inducible nitric oxide synthase. Induces apoptosis in mouse thymus and swine lymphocytes	description Stimulates the activity of inducible nitric oxide synthase.	description purified by gel-filtration chromatography
form lyophilized	form lyophilized	form lyophilized	form lyophilized powder
manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename Calbiochem^®	manufacturer/tradename -
Quality Level 100	Quality Level 100	Quality Level 100	Quality Level 200
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
solubility water: 1 mg/mL	solubility soluble (H2O (5 mg/ml) or 0.5% triethylamine (1 mg/ml). To achieve complete solubilization, heating solution to 37°C with intermittent vortexing or sonication may be necessary.)	solubility water: 5 mg/mL	solubility water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow
storage condition OK to freeze	storage condition OK to freeze	storage condition OK to freeze	storage condition -

Pictograms

GHS06

Signal Word

Danger

Hazard Codes

H300

Precautionary Statements

P264 - P270 - P301 + P310 - P405 - P501

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

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