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100500C

Avanti

E. coli Extract Total

Avanti Research™ - A Croda Brand

Synonym(s):

E. coli Total Lipid Extract

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About This Item

CAS Number:
1240502-50-4
UNSPSC Code:
12352211
NACRES:
NA.25

form

chloroform solution

packaging

pkg of 2 × 20 mL (100500C-1g)
pkg of 1 × 4 mL (100500C-100mg)
pkg of 5 × 4 mL (100500C-500mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

concentration

25 mg/mL (100500C-100mg)
25 mg/mL (100500C-1g)
25 mg/mL (100500C-500mg)

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total is suitable:
  • for solubilization of His6-tagged wtABCG2 and its steroid-binding element (SBE) mutant variants
  • to prepare E. coli liposomes for liposomal content release assay
  • for the determination of the phospholipid types bound to purified AdiC in DDM (n-dodecyl-β-D-maltopyranoside) and OM (n-octyl-β-D-maltopyranoside)

E. coli extract total has been used for the preparation of liposomes. It has also been used to characterize the ternary complexes obtained after purification of membrane proteins in different detergents.
E. coli Extract Total has been used:
  • in the preparation of small unilamellar vesicles (SUVs)
  • as a reference standard in electrospray ionization tandem mass spectrometry (ESI-MS/MS)
  • in the reconstitution of ATP-binding cassette (ABC) transporter for electron paramagnetic resonance spectroscopy studies

Biochem/physiol Actions

The E.coli lipid extract contains phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in the identification and taxonomic grouping of any bacterial sample. It is also used for preparing supported lipid bilayers.

Packaging

30 mL Amber Narrow Mouth Glass Bottle with Screw Cap (100500C-1g)
5 mL Clear Glass Sealed Ampule (100500C-100mg)
5 mL Clear Glass Sealed Ampule (100500C-500mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

also commonly purchased with this product

Product No.
Description
Pricing

Pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system, Liver,Kidney

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Regulation of the Function of the Human ABCG2 Multidrug Transporter by Cholesterol and Bile Acids: Effects of Mutations in Potential Substrate and Steroid Binding Sites
Telbisz A, et al.
Drug Metabolism and Disposition, 42(4), 575?585-575?585 (2014)
Variation of the Detergent-Binding Capacity and Phospholipid Content of Membrane Proteins When Purified in Different Detergents
Ilgu H, et al.
Biophysical Journal, 106, 1660-1670 (2014)
GhoT of the GhoT/GhoS toxin/antitoxin system damages lipid membranes by forming transient pores
Kim JS, et al.
Biochemical and biophysical research communications, 467-472 (2018)
In vitro systems for Atg8 lipidation
Zens B, et al.
Methods, 75, 37-43 (2015)
Variation of the detergent-binding capacity and phospholipid content of membrane proteins when purified in different detergents
Ilgu H, et al.
Biophysical Journal, 106(8), 1660-1670 (2014)
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