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G005613

HPLC Analysis of Withania Extract on Ascentis® Express F5

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 20 to 100% B in 5 min; held at 100% B for 0.5 min
flow rate: 0.3 mL/min
pressure: 2750 psi (190 bar)
column temp.: 35 °C
detector: UV, 227 nm
injection: 5 μL
sample: 20 mg/mL in water:acetonitrile (50:50); sonicate for 15 minutes; filter 0.45 μm; dilute to water:acetonitrile (80:20)

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express F5 for the efficient separation of withania extract.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Withanoside IV
    Withanoside V
    Withaferin A
    12-Deoxywithastramonolide
    Withanolide A
    Withanone
    Withanolide B

analytical column

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Description
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standard

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