V9264

Anti-Vinculin antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

• Synonym(s): Monoclonal Anti-Vinculin antibody produced in mouse
• MDL number: MFCD00162905
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: hVIN-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen 116 kDa
• species reactivity: frog, chicken, mouse, canine, human, bovine, rat, turkey
• packaging: antibody small pack of 25 μL
• concentration: ~1.0 mg/mL
• technique(s): immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
• isotype: IgG1
• UniProt accession no.: P18206
• application(s): research pathology
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

Description

General description

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

Specificity

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

Immunogen

purified human vinculin from uterus.

Application

Anti-Vinculin antibody, Mouse monoclonal has been used in

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves