T2780

Monoclonal Anti-Tropomyosin antibody produced in mouse

clone TM311, ascites fluid

• Synonym(s): Anti-Tropomyosin Antibody
• MDL number: MFCD00149029
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: TM311, monoclonal
• mol wt: antigen 36-39 kDa
• contains: 15 mM sodium azide
• species reactivity: pig, chicken, bovine, rabbit, mouse, hamster, rat, human
• technique(s): immunoprecipitation (IP): suitable; indirect ELISA: suitable; indirect immunofluorescence: 1:400 using chicken fibroblasts; microarray: suitable; western blot: suitable using human tissue and chicken gizzard extracts
• isotype: IgG1
• UniProt accession no.: P09493
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: bovine ... TPM1(281544) ; chicken ... TPM1(396366) ; human ... TPM1(7168) ; mouse ... Tpm1(22003) ; rat ... Tpm1(24851)

Description

General description

Monoclonal Anti-Tropomyosin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tropomyosin is a rigid rod-shaped protein closely associated with actin filaments. Non-muscle forms of tropomyosin have been identified in a wide range of cell types. Tropomyosin is made of two α helical polypeptide chains.

Immunogen

chicken gizzard tropomyosin.

Application

Monoclonal Anti-Tropomyosin has been used:

• in immunofluorescent labelling
• in immunohistochemistry
• in western blotting
• in immunoblotting

Mouse monoclonal clone TM311 anti-Tropomyosin antibody is used to tag tropomyosin for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting, immunoprecipitation, and immunofluorescence. It is used as a probe to determine the presence and roles of tropomyosin in cytoskeletal structures.

Biochem/physiol Actions

Tropomyosin together with troponin, regulate the binding of myosin to actin. Tropomyosin is a dimeric coiled-coil protein forming continuous polymers along the myosin-binding groove of actin. Various tropomyosin isoforms help to confer structure and function to actins in the cytoskeleton and in striated muscle function.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable