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R8903

Sigma-Aldrich

Monoclonal Anti-hnRNP-K/J antibody produced in mouse

clone 3C2, purified immunoglobulin, buffered aqueous solution

Synonym(s):

Anti-Heterogeneous Nuclear Ribonucleoprotein K/J

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About This Item

MDL number:
MFCD06798704
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3C2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

hamster, Xenopus, bovine, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells

isotype

IgG2b

UniProt accession no.

P61978

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... HNRNPK(3190)

General description

Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.

Immunogen

hnRNPs purified by oligo (dC) affinity chromatography.

Application

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

Biochem/physiol Actions

Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Heterogeneous nuclear ribonucleoprotein K upregulates the kinetochore complex component NUF2 and promotes the tumorigenicity of colon cancer cells
Sugimasa H, et al.
Biochemical and Biophysical Research Communications, 459(1), 29-35 (2015)
hnRNP K: One protein multiple processes
Bomsztyk K, et al.
Bioessays, 26(6), 629-638 (2004)
A Proteomic Approach To Identify Candidate Substrates of Human Adenovirus E4orf6-E1B55K and Other Viral Cullin-Based E3 Ubiquitin Ligases
Dallaire F, et al.
Journal of Virology, 83(23), 12172-12184 (2009)
SMN interacts with a novel family of hnRNP and spliceosomal proteins
Mourelatos Z, et al.
The Embo Journal, 20(19), 5443-5452 (2001)
Cheng Zhang et al.
Arteriosclerosis, thrombosis, and vascular biology, 27(8), 1760-1767 (2007-05-05)
Inflammation is known to contribute to the pathogenesis of vascular diseases in which arterial wall extracellular matrix (ECM) homeostasis is disrupted. Tumor necrosis factor-alpha (TNF-alpha), a pivotal cytokine that regulates ECM metabolism by increasing degradation and decreasing production of arterial

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