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O8137

Sigma-Aldrich

Osteoprotegerin/Fc Chimera from mouse

>95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

Synonym(s):

OCIF, OPG

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About This Item

MDL number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.32

biological source

mouse

Quality Level

recombinant

expressed in NSO cells

Assay

>95% (SDS-PAGE)

form

lyophilized powder

potency

25-50 ng/mL ED50

mol wt

100 kDa by SDS-PAGE (reducing)
calculated mol wt 70.9 kDa

packaging

pkg of 100 μg

impurities

endotoxin, tested

UniProt accession no.

storage temp.

−20°C

SMILES string

CCCCCCCCCCC#CCCCCCC(O)=O

InChI

1S/C18H32O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-18(19)20/h2-10,13-17H2,1H3,(H,19,20)

InChI key

XXUPLYBCNPLTIW-UHFFFAOYSA-N

Gene Information

General description

Osteoprotegerin (OPG) is a member of the TNFR (tumor necrosis factor receptor) superfamily that can act as a decoy receptor for RANKL (receptor activator of nuclear factor κ-B ligand) and TRAIL (tumor necrosis factor-related apoptosis-inducing ligand). The primary structure of OPG protein consists of seven distinct domains, four of which corresponds to the extracellular cysteine-rich domains of TNFR proteins and constitutes the soluble OPG. OPG is expressed in a wide variety of tissues including adult heart, lung, kidney and liver. It is secreted both as a monomeric and a dimeric protein.

Biochem/physiol Actions

Binding of soluble osteoprotegerin (OPG) to sRANKL (soluble receptor activator of nuclear factor κ-B ligand) inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. Changes in the level of serum OPG are associated with atherosclerosis and cardiovascular disorders. It might also be associated with malignancy and plays a regulatory role in multiple myeloma, prostate, breast, bladder and gastric carcinoma. OPG plays a vital role in bone metabolism and is involved in inhibition of ovariectomy-associated bone loss in rats.
OPG is a soluble secreted member of the tumor necrosis factor receptor superfamily that lacks any apparent cell-association motifs. It regulates the formation of osteoclasts and is involved in the regulation of bone resorption and remodeling. Human and mouse OPG share approximately 84% and 94% amino acid sequence identity, respectively, with rat OPG.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to neutralize apoptosis of mouse L-929 cells treated with 50 ng/ml recombinant human TRAIL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Polymorphisms in three genes are associated with hemorrhagic stroke.
Liu W
Brain and Behavior, 5(11), e00395-e00395 (2015)
Osteoprotegerin: a novel secreted protein involved in the regulation of bone density.
Simonet WS
Cell, 89(2), 309-319 (1997)
Osteoprotegerin secreted by inflammatory and invasive breast cancer cells induces aneuploidy, cell proliferation and angiogenesis.
Goswami S and Sharma-Walia N
BMC Cancer, 15:935 (2015)
Functions of RANKL/RANK/OPG in bone modeling and remodeling.
Boyce BF and Xing L.
Archives of Biochemistry and Biophysics, 473(2), 139-146 (2008)

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