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A fluorescence multiwell plate reader is suggested for measuring neutrophil elastase using this kit. However, it has not been tested directly on serum samples. In theory, it could work as long as the serum sample contains active elastase, but this may require some research and development to adapt the kit for this purpose. It's important to note that the kit is primarily designed for elastase from neutrophils, which are a type of white blood cell, and serum should be devoid of white blood cells due to the collection process. As for dilutions, it would be advisable to start with a dilution series of the serum sample to determine the optimal concentration for detection.
MAK246
Neutrophil Elastase Activity Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Synonym(s):
Elastase Enzyme Activity Fluorescent Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.25
Pricing and availability is not currently available.
Recommended Products
detection method
fluorometric
relevant disease(s)
hematological disorder; pulmonary disorders
storage temp.
−20°C
General description
Neutrophil Elastase (NE, EC 3.4.21.37, leukocyte elastase, ELANE, ELA2, elastase 2, neutrophil elaszym, or serine elastase) is a cytotoxic serine protease. It is stored in the azurophil granules of neutrophil granulocyte and is released following cell stimulation, e.g. by pathogens, immune complexes or chemotactic agents (PMA). Mutation in the gene ELA2 (neutrophil elastase) results in neutropenia, a hereditary disorder characterized by reduced neutrophil count in an individual.[1]
Application
- Relationship between airway inflammation and airflow limitation in elderly asthmatics.: This study investigates the connection between airway inflammation and airflow limitation in elderly asthmatic patients. It highlights the role of neutrophil elastase in airway inflammation, which was measured using the Neutrophil Elastase Activity Assay Kit (Fluorometric). The findings suggest that targeting neutrophil elastase activity could help in managing airflow limitation in elderly asthmatics, offering potential therapeutic applications for the assay kit (Soma et al., 2020).
Suitability
Suitable to determine the activity of purified Neutrophil Elastase, detect the activity of Neutrophil Elastase in plasma and/or blood and screen/study/characterize chemotactic agents causing Neutrophil stimulation and release of Elastase.
Principle
The Neutrophil Elastase Activity Assay Kit utilizes the ability of NE to proteolytically cleave a synthetic substrate and release a fluorophore, AFC, which can be easily quantified by fluorescence. This assay kit is simple, rapid, and can detect as low as 1 ng of neutrophil elastase in a variety of samples.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
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Neutrophil elastase in cyclic and severe congenital neutropenia.
Horwitz M S, et al.
Blood, 109(5), 1817-1824 (2007)
Sang-Il Han et al.
Bioresource technology, 302, 122791-122791 (2020-01-26)
The effect of co-cultivation of Porphyridium cruentum UTEX 161 with Pseudoalteromonas sp. MEBiC 03485 on P. cruentum growth and its sulfated polysaccharide (EPS) production were examined. The strain MEBiC 03485 had beneficial effects on P. cruentum growth, EPS production, and
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Hi, I want to know what plate reader to use to measure neutrophil elastase and I'm using mouse serum for this and any suggestion on dilutions?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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will this kit work with human and mice sample both?
1 answer-
This kit can be used to determine neutrophil elastase activity from both species. Please review the article citations below where researchers have reported this kit being used with neutrophils isolated from human blood and mice brain.
Pia Kuschnerow, Antje Munder, Nicole de Buhr, Matthias Mörgelin, Adan Chari Jirmo, Mania Ackermann, Maren von Köckritz-Blickwede, Burkhard Tümmler, Nina Cramer,
Competitive survival of clonal serial Pseudomonas aeruginosa isolates from cystic fibrosis airways in human neutrophils, iScience, Volume 26, Issue 4, 2023, 106475, ISSN 2589-0042, https://doi.org/10.1016/j.isci.2023.106475.Li F, Zhang Y, Li R, Li Y, Ding S, Zhou J, Huang T, Chen C, Lu B, Yu W, Boltze J, Li P, Wan J. Neuronal Serpina3n is an endogenous protector against blood brain barrier damage following cerebral ischemic stroke. J Cereb Blood Flow Metab. 2023 Feb;43(2):241-257. doi: 10.1177/0271678X221113897. Epub 2022 Dec 1. PMID: 36457151; PMCID: PMC9903218.
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Hi I am wondering if you have any references where this assay has been used with Human serum. Please let me know. Thanks.
1 answer-
A detailed search of the scientific literature does not reveal any references to utilizing this kit with human serum. This kit has not been tested directly on serum samples. In theory, it could possibly work as long as the serum sample has active elastase. This may require some research and development to utilize the kit for the intended purpose. Please also keep in mind that the kit is used primarily for elastase from neutrophils which is a type of white blood cell. The serum should be devoid of white blood cells from the collection process.
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