The Glutamate Assay Kit (MAK004) has a lower limit of detection of 40 µM and shows a linear standard curve up to 200 µM glutamate. One kit is designed to measure up to 100 samples.
MAK004
Glutamate Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
L-Glutamate Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
neurological disorders; cancer
storage temp.
−20°C
General description
Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism.[1] The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen.[2] Within the mammalian nervous system, glutamate is the most abundant excitatory neurotransmitter.[1] It is believed to be involved in learning and memory and may also be involved in diseases like amyotrophic lateral sclerosis, autism, some forms of mental retardation, and Alzheimer′s disease.[3] Glutamate is present in a wide variety of foods, and has been used as a flavor enhancer in food industry.
Application
Glutamate Assay Kit has been used to determine the concentration of glutamate in cerebrospinal fluid in a rat subarachnoid hemorrhage model.[4] It has also been used to determine cytosolic and secreted glutamate concentrations in cerebrospinal fluid of patients with amyotrophic lateral sclerosis.[5]
Suitability
Suitable to determine glutamate concentration in a variety of samples.
Principle
Glutamate concentration is determined by an enzymatic assay, which results in a colorimetric (450 nm) product, proportional to the glutamate present.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B
Storage Class Code
8A - Combustible corrosive hazardous materials
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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
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Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.
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このキットで定量できるグルタミン酸の濃度の範囲はどれくらいでしょうか？ また、1キットあたり何サンプル分の測定が可能でしょうか？
1 answer-
Helpful?
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Why are cloudy white precipitates sometimes observed after reconstituting the enzyme mix in the Glutamate Assay Kit (MAK004)? Is there a way to reconstitute it?
1 answer-
The presence of precipitates in the enzyme mix may be attributed to the reconstitution temperature. It is suggested to ensure complete equilibration of the Assay Buffer at room temperature before use. Reconstitute the Enzyme Mix according to the protocol using 220 µL Assay Buffer and keep it on ice during the assay. Gently vortexing is also an option. It is noted that the precipitates in the Enzyme Mix should not affect the assay, given that the mix is more concentrated than necessary. Care should be taken to avoid pipetting the precipitates into the wells. However, occasional precipitates in the Enzyme Mix should not have a detrimental impact.
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What is the reason for the lack of color change in the samples at 100 mM concentration, despite the expectation of a rapid or strong color change if the glutamate concentration is out of the standard range?
1 answer-
The standard prepared at 10 nmole/well corresponds to 200 uM. To calculate this, you can use the formula M1V1 = M2V2 based on the preparation in the bulletin. The highest point standard provided in the kit is 200 uM. If the concentration exceeds the range of the standard curve, extrapolating the information for a decision is not appropriate. Dilution is necessary to ensure the concentration is within the standard curve for a correct determination of the glutamate concentration. A concentration of 100 mM is significantly out of range and may be causing the "Hook effect," leading to no color development. The "Hook effect" occurs due to the saturation of the reagents involved in the reaction for color production.
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