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L5283

Sigma-Aldrich

Leukemia Inhibitory Factor human

LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution (pH 7.4), suitable for cell culture

Synonym(s):

Human LIF, Human Leukemia Inhibitory Factor, hLIF, LIF, rLIF

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About This Item

MDL number:
MFCD00163950
UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

buffered aqueous solution (pH 7.4)

potency

0.1-1.5 ng/mL ED50/EC50

quality

endotoxin tested

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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Biochem/physiol Actions

Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline containing 0.02% TWEEN® 20.

Analysis Note

The proliferative activity of human LIF is measured in culture using the human leukemic cell line, TF-1.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Pluripotent stem cell-derived models of neurological diseases reveal early transcriptional heterogeneity.
Sorek, et al.
Genome Biology, 22, 73-73 (2021)
A Rodríguez et al.
Theriogenology, 67(5), 1092-1095 (2007-01-11)
Leukemia inhibitory factor (LIF) is a cytokine that shows conflicting effects on in vitro produced (IVP) bovine embryos. Bovine LIF (bLIF) has been cloned and used in culture, but there is no commercially available bLIF. Thus, researchers use human LIF
Arno Germond et al.
Analytical chemistry, 92(22), 14915-14923 (2020-10-29)
Monitoring cell-state transition in pluripotent cells is invaluable for application and basic research. In this study, we demonstrate the pertinence of noninvasive, label-free Raman spectroscopy to monitor and characterize the cell-state transition of mouse stem cells undergoing reprogramming. Using an
Jin Gyoung Jung et al.
Biology of reproduction, 76(1), 173-182 (2006-10-13)
We recently succeeded in inducing germline transmission by transferring chicken testicular cells into heterologous testes. This study was designed subsequently to identify pluripotent cells in the testicular cells, which would induce the germline transmission. Testicular cells retrieved from juvenile (4-wk-old)
Shigeo Saito et al.
Methods in molecular biology (Clifton, N.J.), 329, 59-79 (2006-07-19)
We describe here the isolation and maintenance of pluripotent embryonic stem (ES) cells from equine blastocysts that have been frozen and thawed. Equine ES cells appear to maintain a normal diploid karyotype in culture. These cells express markers that are
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