D8638

Dodecyltrimethylammonium bromide

≥98%

• Synonym(s): Lauryltrimethylammonium bromide
• Linear Formula: CH₃(CH₂)₁₁N(CH₃)₃Br
• CAS Number: 1119-94-4
• Molecular Weight: 308.34
• Beilstein: 3597463
• EC Number: 214-290-3
• MDL number: MFCD00011767
• UNSPSC Code: 12161900
• PubChem Substance ID: 24894108
• NACRES: NA.25

Properties

• biological source: synthetic (oragnic)
• Quality Level: 200
• description: cationic
• Assay: ≥98%
• form: powder
• mol wt: 308.34 g/mol
• mp: 246 °C (dec.) (lit.)
• solubility: 954 g/L at 20 °C
• density: 1.17 g/cm³ at 20 °C (68 °F)
• SMILES string: [Br-].CCCCCCCCCCCC[N+](C)(C)C
• InChI: 1S/C15H34N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16(2,3)4;/h5-15H2,1-4H3;1H/q+1;/p-1
• InChI key: XJWSAJYUBXQQDR-UHFFFAOYSA-M

Description

General description

Dodecyltrimethylammonium bromide (DTAB) is a versatile quaternary ammonium surfactant with a wide range of applications in cell biology and biochemical research. Its unique properties make it a valuable tool for solubilizing proteins and peptides, extracting DNA, and lysing cells. In proteomics research, DTAB plays a crucial role in protein solubilization and purification. Its ability to disrupt hydrophobic interactions and electrostatic forces allows it to effectively solubilize membrane-bound proteins and peptides, making them accessible for analysis and characterization. This property is particularly important in proteomics studies involving membrane proteins, which are often difficult to solubilize using conventional methods.

Beyond protein solubilization, this cationic detergent also finds application in DNA extraction procedures. Its ability to interact with DNA and alter its conformation facilitates the release of DNA from cellular components, enabling its subsequent purification and analysis. This property is particularly useful in the isolation of DNA from various biological samples, including tissues, cells, and microorganisms. Furthermore, DTAB demonstrates effectiveness in cell lysis processes. Its ability to disrupt cell membranes and release intracellular components makes it a valuable tool for breaking down cells and accessing their contents. This property has applications in various biological studies, including protein extraction, enzyme assays, and cellular component analysis.

Dodecyltrimethylammonium bromide (DTAB) stands as a versatile and widely applicable surfactant with a diverse range of applications in proteomics research, cell biology, and biochemical research. Its ability to solubilize proteins and peptides, extract DNA, and lyse cells makes it an essential tool for various biological studies and analytical techniques.

Application

Dodecyltrimethylammonium bromide has been used in a study to assess the distribution of binary mixed counterions in surfactant adsorbed films. [1] It has also been used in a study to investigate chain length compatibility of surfactants for extraction of organophosphorous pesticides. [2]

Dodecyltrimethylammonium bromide has been used to lyse neonatal normal human epidermal keratinocyte (NHEK) cells for the quantification of intracellular nicotinamide adenine dinucleotide phosphate (NADP⁺) and NADPH (reduced form) content.

Features and Benefits

High-quality compound for your cell biology and biochemical research

Other Notes

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Safety Information

• Pictograms: GHS06,GHS09
• Signal Word: Danger
• Hazard Statements: H301,H315,H319,H410
• Precautionary Statements: P264 - P270 - P273 - P301 + P310 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves