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Key Documents

143B

91112502, human bone, Not specified

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About This Item

UNSPSC Code:
41106514

product name

143B, 91112502

biological source

human bone

growth mode

Adherent

karyotype

Not specified

morphology

Not specified

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human bone osteosarcoma TK-

Cell Line Description

Cells are thymidine kinase deficient (TK-) and resistant to BUdR.143B and HOS (Sigma Catalogue number 87070202) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+.

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 11
vWA: 18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 15ug/ml Bromo-deoxy Uridine (BUdR) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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