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C2409

Creatinase from microorganisms

lyophilized powder, ≥4 units/mg solid

Synonym(s):

Creatine Amidinohydrolase

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About This Item

CAS Number:
37340-58-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
253-471-1
MDL number:
MFCD00130875
EC Number:
3.5.3.3 (BRENDA, IUBMB)
Specific activity:
≥4 units/mg solid
Biological source:
bacterial (Actinobacillus spp.)
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biological source

bacterial (Actinobacillus spp.)

Quality Segment

form

lyophilized powder

specific activity

≥4 units/mg solid

mol wt

~100 kDa

storage temp.

−20°C

General description

Creatinase is a homodimer that catalyzes hydrolysis of creatine. It consists of two monomer subunits and two defined domains; N and C terminal domains. The C-terminal fold has both the α helices and anti-parallel β sheet within two structurally similar domains.In between these two domains, a sulfhydryl group acts as active site, and the activity is metal-independent.
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 1.9 x 10‾2M (Creatine)
Structure: 2 subunits per mole of enzyme
Inhibitors: Cu++, Hg++, Ag+
Optimum pH: 8.0
Optimum temp: 40°C
pH Stability: pH 5.5 − 9.0 (25°C, 16hr)
Thermal stability: Below 50°C (pH 7.5, 30 min)

Application

Creatinase mixed with sarcosine oxidase may be used to determine the level of creatine in different pH, temperature, enzyme ratio, and buffer concentration. It may also be used to determine the plasma creatinine level by using a centrifugal analyser.

Biochem/physiol Actions

Creatinase accelerates the conversion reaction of creatine and water molecule to sarcosine and urea. It always acts in homodimer state and is induced by choline chloride.

Physical form

Lyophilized powder containing sugars and EDTA as stabilizers

Other Notes

One unit will hydrolyze 1.0 μmole of creatine to urea and sarcosine per min at pH 7.5 at 37 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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