A7064

Anti-Biotin−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

• Synonym(s): Anti-Biotin
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d), IHC (p)
• Citations: 6

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous glycerol solution
• technique(s): direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Biotin is a carboxylase co-enzyme that regulates the metabolism of amino acids, glucose and fatty acids. This co-enzyme has been implicated in processes such as immune responses, cell division and fetal growth. Biotin may also be involved in the epigenetic modifications of chromatin.
Goat Anti-Biotin-Alkaline Phosphatase antibody is specific for biotin when tested against biotin-goat IgG, goat IgG, and KLH.

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Immunogen

Biotin-KLH conjugate

Application

ELISA Assays using human spleen cells were performed using alkaline phosphatase-conjugated goat anti-biotin as the secondary antibody with incubation for 1.5 hours at room temperature. Purified IgG from K/BxN, BxSB or C57BL/6 mouse serum was analyzed by western blot using alkaline phosphatase-conjugated goat anti-biotin antibody as the secondary.

Goat Anti-Biotin-Alkaline Phosphatase antibody has been used for dot blot assays. The product can also be used for ELISA (1:30,000) and immunohistochemistry (1:50).

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

May be substituted for avidin to decrease non-specific binding.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)