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Key Documents

0120214

Millipore

Buffered Peptone Water

according to ISO, GB, liquid, bag of 4 × 3 L, sterile

Synonym(s):

BPW in bags, Peptone water (Buffered), Buffered Peptone Water in Bags

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.74

Agency

GB
ISO 6579
ISO 6887
according to GB 4789.30-2016
according to GB 4789.40-2016
according to ISO 21528-1:2017
according to ISO 22964:2017
according to ISO

Quality Level

sterility

sterile

form

liquid

shelf life

limited shelf life, expiry date on the label

feature

ready-to-use

composition

casein peptone, 10 g/L
di-sodium hydrogen phosphate 12H2O, 9 g/L
potassium dihydrogen phosphate, 1.5 g/L
sodium chloride, 5 g/L

packaging

bag of 4 × 3 L

technique(s)

microbiological culture: suitable

final pH

7.0±0.2 (25 °C)

application(s)

environmental
food and beverages
industrial qc
microbiology
pathogen testing
sample preparation

suitability

Citrobacter ssp.
Cronobacter ssp.
Escherichia coli
Klebsiella ssp.
Proteus ssp.
Providencia ssp.
Pseudomonas ssp.
Salmonella spp.
Shigella ssp.
Staphylococcus ssp.
Streptococcus ssp.
Yersinia ssp.
bacteria
coliforms
enterobacteriaceae

General description

Buffered peptone water in bags is a pre-enrichment medium designed to help the recovery of sub-lethally damaged microorganisms before transferring to a selective medium. This pre-enrichment medium is free from inhibitors and is well buffered and provides conditions for resuscitation of the cells that have been injured by processes of food preservation. The media contains Peptone including an enzymatic digest of casein as a source of carbon, nitrogen, vitamins, and minerals. Sodium chloride maintains the osmotic balance and phosphates buffer the medium. The broth is rich in nutrients and produces high resuscitation rates for sub-lethally injured bacteria and supports intense growth.

Application

Buffered Peptone Water is used as :
  • a non-selective pre-enrichment medium for the detection of Salmonella in food products and environmental specimens. It complies with the standard EN ISO 6579 (1) and amendment A1 (annex D) (8),
  • a diluent for the enumeration of the micro-organisms. It complies with the standards EN ISO 6887 (2, 3, 4, 5) and 8261 (6),
  • a diluent for the enumeration of Listeria monocytogenes. It complies with the standard EN ISO 11290-2 (7).

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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