HSMGTKB

KAPA HotStart Mouse Genotyping Kit

• Synonym(s): genotyping, mouse genotyping
• UNSPSC Code: 41106300
• NACRES: NA.55

Properties

• usage: sufficient for 100 reactions (KK7351); sufficient for 100 reactions; sufficient for 500 reactions (KK7352); sufficient for 500 reactions
• Quality Level: 100
• shelf life: ≤18 mo.
• feature: dNTPs included; hotstart
• manufacturer/tradename: Roche
• technique(s): PCR: suitable; genotyping: suitable
• input: crude DNA
• storage temp.: −20°C

Description

General description

KAPA HotStart^® Mouse Genotyping Kits include KAPA Express Extract, a novel thermostable protease and buffer system that allows for the extraction of polymerase chain reaction (PCR)-ready DNA from mouse tissue in less than 15 minutes, and KAPA2G Fast Genotyping Mix with dye, containing a DNA polymerase engineered via directed evolution for high processivity and extreme speed. The combination of KAPA Express Extract and KAPA2G Fast Genotyping Mix allows for the reliable extraction and amplification of DNA fragments from mouse tissue in as little as 1 hour, as compared to ≥1 day with conventional protocols. KAPA HotStart Mouse Genotyping Kit is designed for rapid extraction and amplification of DNA from mouse tissue.

Application

KAPA HotStart^® Mouse Genotyping Kit has been used for:

• extraction and amplification from mouse tail, ear, and toe tissue
• extraction and amplification of DNA from other animal tissue

Features and Benefits

Increased throughput, turnaround time and reliability

• PCR-ready DNA generated in as little as 15 minutes.
• Minimal handling, thereby reducing the risk of sample loss or contamination.
• Sufficient template for multiple assays; easily scaled to handle samples in a 96-well format.
• Fast and reliable amplification of DNA fragments across a wide range of amplicon lengths and GC contents.

Outperforms crude extraction methods and commercial kits

• Increased turnaround times
• Increased specificity
• Decreased cost

Quick Notes:

• Extract PCR-ready DNA from mouse tail, ear, or toe tissue in 15 minutes.
• Dilute DNA extract 10-fold prior to PCR in 10 mM Tris-HCl (pH 8.0–8.5), and use 1 μL of DNA per 25 μL reaction.
• Extracts are stable at -20°C for >6 months.
• KAPA2G Fast (HotStart) Genotyping Mix contains the engineered KAPA2G Fast DNA Polymerase for fast PCR, along with buffer, MgCl₂ (1.5 mM at 1X), and dNTPs (0.2 mM each at 1X).
• Loading dye for electrophoresis is included in the KAPA2G Fast Genotyping Mix, which allows direct loading of PCR products onto agarose gels for analysis.
• Use 15 sec annealing time, with 15 sec extension time for amplicons <1 kb, or 15–30 sec/kb extension for longer amplicons.

Quality

All components contained with KAPA HotStart Mouse Genotyping Kits are subjected to stringent quality control tests, are free of contaminating exo and endonuclease activities and meet strict requirements with respect to DNA contamination.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days).

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Kit Components Only

• KAPA Express Extract Enzyme 1 U/µL
• 10X KAPA Express Extract Buffer
• 2X KAPA2G Fast HotStart^® Genotyping Mix with dye

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H371
• Precautionary Statements: P260 - P264 - P270 - P308 + P311 - P405 - P501
• Hazard Classifications: STOT SE 2
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash