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MAB364

Sigma-Aldrich

Anti-MAP2A, 2B, 2C Antibody, clone HM-2

ascites fluid, clone HM-2, Chemicon®

Synonym(s):

Anti-MAP-2, Anti-MAP2A, Anti-MAP2B, Anti-MAP2C

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

HM-2, monoclonal

species reactivity

bovine, human, quail, rat, chicken, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MAP2(4133)

Specificity

Shows selective staining of dendritic trees throughout the brain in immunohistochemical staining of brain tissue. In perkinje neurons, staining is restricted to the dendrites distal to the initial dendrite segment. No staining of cell body in mature Perkinje cells but strong staining of cell bodies of developing Perkinje cells from neonatal rats. No detectable staining of axons or non-neuronal cells. Reacts specifically with all forms of MAP2 (MAP2a ~280 kDa, MAP2b ~280 kDa and MAP2c ~70 kDa) as determined by immunoblot. No cross-reactivity observed to other MAPs or tubulin.

Immunogen

Rat brain microtubule-associated protein.

Application

Anti-MAP2A Antibody, 2B, 2C, clone HM-2 detects level of MAP2A & has been published & validated for use in WB, IH.
Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation.

Immunohistochemistry: 1:50 to 1:1000

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Physical form

Ascites fluid. Liquid with 15 mM sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T Sorimachi et al.
Neurologia medico-chirurgica, 37(3), 236-242 (1997-03-01)
The protective effect of prior ischemia on focal cerebral infarction produced by permanent middle cerebral artery (MCA) occlusion was studied in a gerbil model. Forebrain ischemia for 2 and 5 minutes at an interval of 48 hours was used as
Jie Gao et al.
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The extensive use of organophosphates (OPs) is an ongoing environmental health concern due to multiple reports of OP-related neurologic abnormalities. The mechanism of the acute toxicity of OPs has been attributed to inhibition of acetylcholinesterase (AChE), but there is growing
Jean G Toma et al.
Stem cells (Dayton, Ohio), 23(6), 727-737 (2005-05-27)
We have previously isolated, expanded, and characterized a multipotent precursor cell from mammalian dermis (termed skin-derived precursors [SKPs]) that can differentiate into both neural and mesodermal progeny. In this study, we report the isolation, expansion, and characterization of a similar
Lauri M Louhivuori et al.
Stem cells and development, 24(6), 701-713 (2014-10-28)
The guidance of developing neurons to the right position in the central nervous system is of central importance in brain development. Canonical transient receptor potential (TRPC) channels are thought to mediate turning responses of growth cones to guidance cues through
Kentaro Takakura et al.
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The genus Cordyceps and its specific ingredient, cordycepin, have attracted much attention for multiple health benefits and expectations for lifespan extension. We analyzed whether Cordyceps militaris (CM), which contains large amounts of cordycepin, can extend the survival of Dahl salt-sensitive

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