05-1416

Anti-CD45 Antibody, clone IBL-5/25

clone IBL-5/25, from rat

• Synonym(s): CD45 associated protein, CD45-associated protein, Lymphocyte phosphatase-associated phosphoprotein, PTPRC-associated protein, protein tyrosine phosphatase, receptor type, C-associated protein, protein tyrosine phosphatase, receptor type, c ; ; polyp
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: IBL-5/25, monoclonal
• species reactivity: mouse
• technique(s): flow cytometry: suitable; immunohistochemistry: suitable
• isotype: IgG
• NCBI accession no.: NP_002829.2
• UniProt accession no.: Q14761
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Ptprc(19264)

Description

General description

CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

Specificity

Reactivity with other species has not been determined.

This antibody reacts with all isoforms of mouse CD45 which is expressed on all haematopoietic cells except erythrocytes.
FUSION PARTNER: SP2/0 myeloma cell line

Immunogen

Mouse lymph node cells.

Application

Anti-CD45 Antibody, clone IBL-5/25 is an antibody against CD45 for use in FC, IH.

Flow Cytometry Analysis: A previous lot detected CD45, clone IBL-5/25 in mouse peripheral blood lymphocytes

Quality

Evaluated by Immunohistochemistry in mouse colon tissue.
Immunohistochemistry Analysis: 1:100 dilution of this antibody detected CD45 in mouse colon tissue.

Target description

Approx. 147 kDa

Linkage

Replaces: CBL1326

Physical form

Format: Purified

Purified rat monoclonal IgG in phosphate buffered saline containing 10 mM sodium azide.

Storage and Stability

Store at 2-8°C for 12 months from date of receipt.

Analysis Note

Control
Mouse colon tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable