SRP5251
PKC-α, active, GST tagged from Xanopus sp.
PRECISIO®, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Synonym(s):
MGC82897, PRKCA
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
biological source
Xenopus sp.
recombinant
expressed in baculovirus infected Sf9 cells
product line
PRECISIO®
Assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
specific activity
186-290 nmol/min·mg
mol wt
~103 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
Xenopus laevis ... prkca(447031)
General description
Protein kinase C-α (PKC-α) is a member of the PKC family of serine- and threonine-specific protein kinases that can be activated by calcium and the secondary messenger diacylglycerol. The protein is expressed in the dorsal ectoderm of Xenopus.
Biochem/physiol Actions
Protein kinase C-α (PKC-α) has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint and cell volume control. It has been identified as a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes.
Physical form
Supplied in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Preparation Note
After opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles.
Legal Information
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
aPKC phosphorylates p27Xic1, providing a mechanistic link between apicobasal polarity and cell-cycle control.
Sabherwal N
Developmental Cell, 31(5), 559-571 (2014)
PKC-alpha regulates cardiac contractility and propensity toward heart failure.
Braz JC
Nature Medicine, 10(3), 248-254 (2004)
Protein kinase C isozymes have distinct roles in neural induction and competence in Xenopus.
Otte AP and Moon RT
Cell, 68(6), 1021-1029 (1992)
Yi Liu et al.
The Biochemical journal, 478(10), 1999-2017 (2021-05-08)
Human hepatic tryptophan 2,3-dioxygenase (hTDO) is a homotetrameric hemoprotein. It is one of the most rapidly degraded liver proteins with a half-life (t1/2) of ∼2.3 h, relative to an average t1/2 of ∼2-3 days for total liver protein. The molecular mechanism
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service