MBD0042

Escherichia coli FISH probe - Cy3

Probe for fluorescence in situ hybridization (FISH)

• UNSPSC Code: 12352200
• NACRES: NA.55

Properties

• Quality Level: 200
• technique(s): FISH: suitable
• fluorescence: λ_ex 550 nm; λ_em 570 nm
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells.1 Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.2

FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms5 and aquatic systems 6) and plants7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control (MBD0034/35) probes, that accompany the specific probe of interest.

Escherichia coli is a gram negative, facultative aerobic, rod-shaped coliform bacterium. E. coli colonizes the infant gut within hours of birth and establishes itself as the most abundant facultative anaerobe of the human intestinal microflora for the remainder of life, equipped with the abilities to grow in the ever-changing environment in the gut and cope with the mammalian host interaction.8,9 Nevertheless, E. coli can survive in many different ecological habitats, including abiotic environments, and is considered a highly versatile species. Known habitats of E. coli include soil, water, sediment, and food. Some strains of E. coli have evolved and adapted to a pathogenic lifestyle and can cause different disease pathologies.10

Escherichia coli probe specifically recognizes Escherichia coli cells.

Yet there are some reports that describe recognition of other bacteria with this probe, such as, Shigella boydii, Citrobacter davisae, Citrobacter lapagei, Citrobacter neteri 11 and Klebsiella pneumoniae 12.

FISH technique was successfully used to identify E.coli with the probe in various samples such as pure culture (as described in the figure legends and11,13), large and small intestines samples14-16, fecal samples17-21, colonic biopsies18, urine samples, bladder and kidney sections embedded in paraffin22 and in E.coli biofilm23.

Application

Probe for fluorescence in situ hybridization (FISH),recognizes Escherichia coli cells

Features and Benefits

• Visualize, identify and isolate Escherichia coli cells.
• Observe native E. coli cell populations in diverse microbiome environments.
• Specific, sensitive and robust identification of E. coli in bacterial mixed population.
• Specific, sensitive and robust identification even when E. coli is in low abundance in the sample.
• FISH can complete PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on E.coli morphology and allows to study biofilm architecture.
• Identify E.coli in clinical samples such as, urine samples, bladder and kidney sections (formalin-fixed paraffin-embedded (FFPE) samples), fecal samples and colon tissue.
• The ability to detect E.coli in its natural habitat is an essential tool for studying host-microbiome interaction.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable