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M3770

Sigma-Aldrich

Micrococcus lysodeikticus ATCC No. 4698

suitable for substrate for the assay of lysozyme, lyophilized cells

Synonym(s):

Micrococcus luetus

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized cells

Quality Level

200

suitability

suitable for substrate for the assay of lysozyme

storage temp.

−20°C

Application

Micrococcus lysodeikticus ATCC No. 4698 has been used in a study to assess lysozyme separation by hollow-fibre ultrafiltration. It has also been used in a study to investigate the encapsulation of protein drugs in biodegradable microparticles.
Lysozyme lysates harvested from cultures of Micrococcus lysodeikticus were attached to sepharose and used for affinity chromatography to isolate various bacteriolytic enzymes.

Biochem/physiol Actions

Micrococcus luetus is a Gram-positive bacteria that is identified by the release of yellow water-insoluble pigments. This species requires succinic acid for its growth and is found to be susceptible to β-lytic metalloendopeptidase lyses by Lysobacter enzymogenes. Its membrane includes enzymes that participate in the prenylation reactions by utilizing prenyl pyrophosphates as donors. M. luteus is known to be used for cloning the cis-prenyl transferase gene.

Quality

Contains polynucleotide phosphorylase.

Unit Definition

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J C Cox et al.
Bioorganic & medicinal chemistry, 9(10), 2525-2531 (2001-09-15)
The in vitro selection of nucleic acid binding species (aptamers) is frequently repetitive, time-consuming, and poorly adapted to high-throughput applications. We have adapted automated workstations to select anti-protein aptamers; as an example, we demonstrated the selection of anti-lysozyme aptamers that
C L Greenblatt et al.
Microbial ecology, 48(1), 120-127 (2004-05-28)
A growing body of evidence now supports the isolation of microorganisms from ancient materials. However, questions about the stringency of extraction methods and the genetic relatedness of isolated organisms to their closest living relatives continue to challenge the authenticity of
Cecilia C Sánchez et al.
BMC genomics, 12, 626-626 (2011-12-23)
Fish under intensive culture conditions are exposed to a variety of acute and chronic stressors, including high rearing densities, sub-optimal water quality, and severe thermal fluctuations. Such stressors are inherent in aquaculture production and can induce physiological responses with adverse
Tania Jauslin et al.
mBio, 12(1) (2021-02-18)
Ingestion and killing of bacteria by phagocytic cells protect the human body against infections. While many mechanisms have been proposed to account for bacterial killing in phagosomes, their relative importance, redundancy, and specificity remain unclear. In this study, we used
Seav-Ly Tran et al.
Toxins, 12(9) (2020-09-18)
The emergence of B. cereus as an opportunistic food-borne pathogen has intensified the need to distinguish strains of public health concern. The heterogeneity of the diseases associated with B. cereus infections emphasizes the versatility of these bacteria strains to colonize
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Protocols

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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