The protein is expressed in E. coli as a GST-tagged fusion protein. After purification, the tag is removed by thrombin treatment to product the final protein sequence.
L5158
Leukemia Inhibitory Factor from mouse
≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous solution, suitable for cell culture
Synonym(s):
Mouse LIF, Mouse Leukemia Inhibitory Factor, Murine LIF, mLIF, LIF
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Product Name
Leukemia Inhibitory Factor from mouse, LIF, recombinant, expressed in E. coli, 10 μg/ml, buffered aqueous solution, suitable for cell culture
recombinant
expressed in E. coli
Quality Level
Assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
quality
endotoxin tested
technique(s)
cell culture | mammalian: suitable
storage temp.
2-8°C
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Application
Leukemia Inhibitory Factor from mouse has been used:
- as a component of enriched co-culture media[1]
- as a supplement in Dulbecco′s modified eagle′s medium (DMEM)/F12 medium for culturing heart cells[2]
- as a component in maintenance medium for maintaining embryonic stem cells[3]
- to treat ovaries in ovarian culture[4]
Biochem/physiol Actions
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein originally described to inhibit the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Other activities were later identified to LIF, which is known by a variety of synonyms, including DIF, D-factor, DIA, DRF, CNDF, HILDA, HSF-III, and MLPLI. Human LIF exerts its actions through a receptor comprising a 190 kDa LIF-binding α-chain (130 kDa, mouse) and a 130 kDa signal-transducing β-chain (gp130), which is shared with CNTF, OSM, L-6 and IL-11. As such, it is a member of the gp130 family of the cytokine receptor superfamily. LIF receptors have been identified on several cells, including monocytes, liver, placenta and embryonic stem cells. Natural LIF is heavily glycosylated, showing an apparent molecular weight of 32 kDa to 62 kDa, depending on the source, but absence of glycosylation appears not to affect its biological activity. A single gene encodes LIF, which is secreted as a single chain glycoprotein containing 180 amino acids for human or mouse with a conserved disulfide bond. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Leukemia Inhibitory Factor (LIF) is a pleiotropic glycoprotein that inhibits the proliferation of the murine myeloid leukemic cell line M1, while inducing differentiation into macrophages. Human and mouse LIF share 78% sequence homology. Human LIF can activate mouse cells, but mouse LIF cannot activate human cells.
Physical form
Solution in phosphate buffered saline containing 0.02% TWEEN® 20
Analysis Note
The proliferative activity of mouse LIF is measured in culture using the M1 murine cell line.[5]
Legal Information
TWEEN is a registered trademark of Croda International PLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Articles
Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).
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How is this recombinant protein, Leukemia Inhibitory Factor, Product L5158, prepared?
1 answer-
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Is Leukemia Inhibitory Factor, Product L5158, the same as the LIF from Chemicon?
1 answer-
Chemicon holds the patent for manufacture of mouse recombinant LIF. The product from Sigma-Aldrich is identical to that from Chemicon.
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What is the sequence of the protein Leukemia Inhibitory Factor, Product L5158?
1 answer-
The partial sequence is reported on the SwissProt website under the accession number P09056. The recombinant has 1 more amino acid but the supplier has kept details as proprietary.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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