I3525

Interleukin-17A human

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): CTLA-8, IL-17
• MDL number: MFCD01094096
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE)
• form: lyophilized powder
• potency: ≤2.0 ng/mL ED₅₀
• mol wt: 31 kDa
• packaging: pkg of 50 μg
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested (endotoxin level is less than 0.1 ng per μg cytokine (1 EU/μg).)
• UniProt accession no.: Q16552
• storage temp.: −20°C
• Gene Information: human ... IL17A(3605)

Description

Biochem/physiol Actions

IL-17, initially known as CTLA-8, is a T cell-expressed polypeptide that appears to be involved in the regulation of the hematopoietic system. IL-17 has been shown to induce IL-6, IL-8, and G-CSF production by fibroblasts; all of these cytokines have demonstrated effects on hematopoiesis.

Physical form

Lyophilized with no additives.

Analysis Note

• The biological activity of human IL-17 is measured by its ability to induce IL-6 production using NHDF cells.
• Biological activity is also determined by the dose-dependent induction of IL-6 in primary human foreskin fibroblast. The expected ED50 is < 2.0 ng/ml, corresponding to a specific activity of > 5 x 105 units/mg.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves