Most known chemiluminescence (CL) reactions exhibit flash-type light emission. Great efforts have been devoted to the development of CL systems that emit light with high intensity and long-lasting time. However, a long-lasting CL system that can last for hundreds of hours
Journal of chromatography, 615(1), 97-110 (1993-05-19)
N-(4-Aminobutyl)-N-ethylisoluminol was used for labelling of carboxylic acids. The derivatization reaction was carried out with 1-hydroxybenzotriazole as pre-activator of the carboxylic acid function and N-ethyl-N'-(3-dimethylaminopropyl)carbodiimide as the coupling reagent. Optimum conditions for the derivatization were determined by using factorial design
Chemical communications (Cambridge, England), 48(5), 699-701 (2011-12-03)
We report on a novel strategy to improve microfluidic immunoassay sensitivity by introducing chemiluminescence resonance energy transfer (CRET) into the immunoreactions. The proposed CRET-based immunoassay for estradiol (E2, as a model analyte) is one of the most sensitive immunoassay with
Journal of immunoassay, 7(3), 169-179 (1986-01-01)
Chemiluminescent labels have been shown to be interesting alternatives to radioisotope labels. Disadvantages of the latter are preparation of e.g. labelled protein/peptides every four to six weeks, and problems with storage and disposal. Amino-Butyl-Ethyl-Isoluminol(ABEI) was attached to the alpha-amino function
A microchip electrophoresis (MCE) method with chemiluminescence (CL) detection was developed for the determination of carnosine-related peptides, including carnosine, homocarnosine, and anserine, in biological samples. A simple integrated MCE-CL system was built to perform the assays. The highly sensitive CL
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