IF002
Interferon-γ Protein, Recombinant human
The Interferon-g protein (or IFN-gamma protein) is a lymphoid factor which possesses potent anti-viral activity.
Synonym(s):
Human Interferon, Human Interferon-γ, Recombinant Interferon-γ
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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.77
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biological source
human
Quality Level
Assay
>98% (SDS-PAGE and HPLC)
specific activity
≥2 x 10(E7) U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
input
sample type hematopoietic stem cell(s)
sample type mesenchymal stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... IFNG(3458)
General description
Interferon-g (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-gamma is a 16.7 kDa protein containing 143 amino acid residues.
Product Source: Human, expressed in E.coli
Application
For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10 ng/mL. Responding cells are (partial list): Lymphocytes.
Linkage
Replaces: IF3
Physical form
Sterile filtered, lyophilized from 10 mM sodium phosphate, pH 7.4.
Storage and Stability
The lyophilized IFN-g, though stable at room temperature, is best stored desiccated at -20°C. After a quick spin, reconstitute in 1x PBS, pH 8.0 to a concentration of 0.5-1.0 mg/mL. Note: Further dilutions should be made into RPMI + 10% FCS or 5% HSA and stored at 4°C for 1 week or -20°C for future use. Reconstituted material should be stored in undiluted aliquots at -20°C for up to six months.
Analysis Note
Specific Activity: Human IFN-gamma is fully biologically active when compared to standards.The ED(50) as determined in a viral resistance assay is ≤ 0.05 ng/mL
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
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