Skip to Content
Merck
All Photos(2)

Documents

16-256

Sigma-Aldrich

Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor™ 488

clone 1H6, Upstate®, from mouse

Synonym(s):

488-Alexa Fluor Antibody, Alexa Fluor 488 Detection, Clone 1H6 Anti-Phosphatidylserine

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

ALEXA FLUOR™ 488

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

1H6, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

phosphorylation (pSer)

General description

Significance:
Anti-Phosphatidylserine may be used to detect translocation of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer cell membrane leaflet; it provides an alternative to Annexin V for quantitation of apoptosis.
Phosphatidylserine, or PS, is a naturally occurring, phospholipid nutrient. PS is essential to the functioning of all the cells of the body, but is most concentrated in the brain. Its relative abundance in this organ reflects its proven involvement in an assortment of nerve cell functions, including nerve transmitter release and synaptic activity. Clinical studies have suggested that PS can support brain functions that tend to decline with age.

Specificity

Recognizes phosphatidylserine (PS) in cell membranes.

Immunogen

Liposomes containing 70% phosphatidylserine and 30% phosphatidylglycerol.

Application

Detect Phosphatidylserine using this Anti-Phosphatidylserine Antibody, clone 1H6, Alexa Fluor 488 validated for use in FC.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Flow Cytometry: 0.2 μg of this antibody detected Phosphatidylserine in fixed Jurkat cells (see page 2).

Apoptosis Assay: Time course for induction of apoptosis in Jurkat cells by staurosporine, measured using either an Anti-Phosphatidylserine, clone 1H6, Alexa Fluor™ 488 conjugate or an Annexin V FITC conjugate.

Physical form

Protein G Purified
Purified mouse monoclonal IgG in buffer containing PBS containing 1% BSA, 0.05% Tween, 0.05% sodium azide. Liquid at 4ºC.

Storage and Stability

Stable for 1 year at from date of receipt.

Analysis Note

Control
Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor™ 488-conjugate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Yi An et al.
Scientific reports, 11(1), 6392-6392 (2021-03-20)
Head and neck squamous cell carcinomas (HNSCC) induced by human papillomavirus (HPV) have increased recently in the US. However, the distinct alterations of molecules involved in the death pathways and drug effects targeting inhibitor of apoptosis proteins (IAPs) have not
Yuta Nakazawa et al.
eLife, 10 (2021-11-10)
Although tumor-infiltrating regulatory T (Treg) cells play a pivotal role in tumor immunity, how Treg cell activation are regulated in tumor microenvironments remains unclear. Here, we found that mice deficient in the inhibitory immunoreceptor CD300a on their dendritic cells (DCs)
Carlos E Bravo-Iñiguez et al.
Nature communications, 14(1), 3122-3122 (2023-06-02)
Deficiency of coagulation factor VIII in hemophilia A disrupts clotting and prolongs bleeding. While the current mainstay of therapy is infusion of factor VIII concentrates, inhibitor antibodies often render these ineffective. Because preclinical evidence shows electrical vagus nerve stimulation accelerates
The role of phosphatidylserine in recognition and removal of erythrocytes.
Kuypers, F A and de Jong, K
Cellular and molecular biology (Noisy-le-Grand, France), 50, 147-158 (2004)
I Vermes et al.
Journal of immunological methods, 184(1), 39-51 (1995-07-17)
In the early stages of apoptosis changes occur at the cell surface, which until now have remained difficult to recognize. One of these plasma membrane alterations is the translocation of phosphatidylserine (PS) from the inner side of the plasma membrane
View All Related Papers

Articles

Flow Cytometry Dye Selection Tips

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Flow Cytometry Dye Selection Tips

Flow cytometry dye selection tips match fluorophores to flow cytometer configurations, enhancing panel performance.

Flow Cytometry Troubleshooting Guide

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

Flow Cytometry Troubleshooting Guide

Troubleshooting guide offers solutions for common flow cytometry problems, ensuring improved analysis performance.

See All

Protocols

Key Steps in Flow Cytometry Protocols

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Flow Cytometry Guide

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Flow Cytometry Guide

Explore our flow cytometry guide to uncover flow cytometry basics, traditional flow cytometer components, key flow cytometry protocol steps, and proper controls.

Key Steps in Flow Cytometry Protocols

Learn key steps in flow cytometry protocols to make your next flow cytometry experiment run with ease.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service