25351
SPB®-5 Capillary GC Column
L × I.D. 60 m × 0.53 mm, df 5.00 μm
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About This Item
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material
fused silica
Quality Level
Agency
meets requirements for USP G27 and G36
parameter
-60-260 °C temperature (isothermal)
-60-280 °C temperature (programmed)
Beta value
27
df
5.00 μm
technique(s)
gas chromatography (GC): suitable
L × I.D.
60 m × 0.53 mm
matrix active group
Bonded; poly(5% diphenyl/95% dimethyl siloxane) phase
column type
capillary non-polar
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General description
Capillary GC column is also known as open tubular column. The carrier gas flows through the central aperture and is unrestricted throughout the length of the column.
Application: This non-polar general purpose column provides primarily a boiling point elution order with a slight increase in selectivity, especially for aromatic compounds.
USP Code: This column meets USP G27 and G36 requirements.
Phase:
USP Code: This column meets USP G27 and G36 requirements.
Phase:
- Bonded
- Poly(5% diphenyl/95% dimethyl siloxane)
- ≤0.32 mm I.D., <2 μm: -60 °C to 320 °C (isothermal or programmed)
- ≤0.32 mm I.D., ≥2 μm: -60 °C to 300 °C (isothermal or programmed)
- ≥0.53 mm I.D., <2 μm: -60 °C to 300 °C (isothermal) or 320 °C (programmed)
- ≥0.53 mm I.D., ≥2 μm: -60 °C to 260 °C (isothermal) or 280 °C (programmed)
Application
SPB®-5 Capillary GC Column was used for speciation of the nitrogen compounds (quantitatively), which are polar and somewhat basic in nature, with chemiluminescence detection. It may be used as stationary phase for determination of nivalenol and deoxynivalenol in cereals by electron-capture gas chromatography. It may also find application in being used as stationary phase in capillary column gas chromatography for determination of sterols.
Other Notes
We offer a variety of chromatography accessories including analytical syringes
Legal Information
SPB is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Adipose tissue response to endocrine stimuli, such as insulin, is crucial for metabolic adaptation at the onset of lactation in dairy cows. However, the exact molecular mechanisms behind this response are not well understood. Thus, the aim of this study
Determination of nivalenol and deoxynivalenol in cereals by electron-capture gas chromatography
Journal - Association of Official Analytical Chemists, 889-893 (1985)
Determination of sterols by capillary column gas chromatography. Differentiation among different types of olive oil: virgin, refined, and solvent-extracted.
Journal of the American Oil Chemists' Society, 73 (12), 1685-1689 (1996)
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Nivalenol (NIV) is an important Fusarium mycotoxin shown to occur naturally in grains from several countries. Extraction and cleanup procedures previously described for trichothecene analysis have been modified to give good recoveries of NIV from cereal products. The extraction solvent
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