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SAB4200383

Sigma-Aldrich

Anti-SMAD6 (C-terminal) antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody

Synonym(s):

Anti-MADH6, Anti-MADH7, Anti-SMAD family member 6

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~60 kDa

species reactivity

mouse, human, rat

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells over-expressing human SMAD6.
indirect immunofluorescence: 10-20 μg/mL using mouse 3T3 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells over-expressing human SMAD6.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... SMAD6(4091)
mouse ... Smad6(17130)
rat ... Smad6(374096)

General description

SMAD family member 6 (SMAD6) is encoded by the gene mapped to human chromosome 15q22.31. The encoded protein is localized in both nuclei and cytoplasm and is expressed in variety of human tissues, including ovary. SMAD6 consists of MAD homology 2 (MH2) domain, involved in protein-protein interaction.

Immunogen

peptide corresponding to the C-terminal region of human SMAD6, conjugated to KLH. The corresponding sequence is identical in rat, mouse and bovine.

Biochem/physiol Actions

SMAD family member 6 (SMAD6) induces endocytosis and negatively regulates apoptosis by inhibiting transforming growth factor-β (TGF-β) signaling pathway. In addition, it also indirectly regulates stemness by inhibiting erythropoiesis in cord blood hematopoietic stem cells (HSCs). Mutation in the gene leads to congenital cardiovascular malformation (CVM). Overexpression or genetic variation of SMAD6 is associated with the development of ovarian cancer.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Genetic variants in TGF-β pathway are associated with ovarian cancer risk.
Yin J, et al.
PLoS ONE, 6(9), e25559-e25559 (2011)
Inhibition of erythropoiesis by Smad6 in human cord blood hematopoietic stem cells.
Kang YJ
Biochemical and Biophysical Research Communications, 423(4), 750-756 (2012)
Nonsynonymous variants in the SMAD6 gene predispose to congenital cardiovascular malformation.
Tan HL
Human Mutation, 33(4), 720-727 (2012)
Statistical genetic analysis of serological measures of common, chronic infections in Alaska Native participants in the GOCADAN study.
Rubicz R
Genetic Epidemiology, 37(7), 751-757 (2013)

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