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MAK262

Sigma-Aldrich

Sphingomyelin Quantification Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

General description

Sphingomyelin, a sphingolipid consisting of a phospholipid (typically phosphocholine) attached to a ceramide moiety, is a major component of cellular membranes. Sphingomyelin is enriched in the exoplasmic leaflet of the cell membrane. Within the cell membrane, sphingomyelin interacts with cholesterol to form lipid rafts. The metabolism of sphingomyelin contributes to cellular signaling via the release of ceramide, a second messenger that participates in multiple cellular signaling pathways. Sphingomyelin accumulates in Niemann-Pick disease due to deficiencies in sphingomyelinase activity. Increased levels of sphingomyelin is observed in hypercholesterolemia, which is characterized by the accumulation of sphingomyelin in arteriosclerotic lesions resulting in ischemic heart disease.

Suitability

Suitable for quantifying sphingomyelin in serum, plasma, and other biological fluids, and tissue and cell culture samples.

Principle

The Sphingomyelin Quantification Assay Kit is a simple and sensitive method for quantifying sphingomyelin. The assay is based on the hydrolysis of sphingomyelin into ceramide and phosphocholine by sphingomyelinase. Alkaline phosphatase (ALP) dephosphorylates phosphocholine to choline producing a reaction that generates a colorimetric signal (A570). The assay is sensitive to 1 M of sphingomyelin in a variety of samples.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Absorption and lipoprotein transport of sphingomyelin.
Nilsson A and Duan R D
Journal of Lipid Research, 47(1), 154-171 (2006)
Jie Xiong et al.
International journal of molecular medicine, 46(3), 1003-1012 (2020-06-26)
Alcoholic liver disease greatly affects human health. Previous studies have identified that microRNAs (miRNAs) are associated with the pathogenesis of alcoholic liver fibrosis (ALF). Therefore, the present study explored the regulatory mechanism of miR‑148a‑3p in ALF. An ALF model was established in

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