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H3662

Sigma-Aldrich

HEPES sodium salt solution

BioReagent, 1M, suitable for cell culture

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
75277-39-3
Molecular Weight:
260.29
MDL number:
MFCD00036463
UNSPSC Code:
12161700
PubChem Substance ID:
24895590
NACRES:
NA.25

Quality Level

200

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
 DNase, RNase. Protease, Nickase, free
 endotoxin, tested
<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Hydrogen ion buffers for biological research.
Good NE, et al.
BioChemistry: An Indian Journal, 5, 467-477 (1996)
I J Chopra et al.
Thyroid : official journal of the American Thyroid Association, 6(4), 255-259 (1996-08-01)
We have devised a practical, sensitive, and reliable assay for measurement of free T3 concentration in serum. The assay employs a convenient and disposable plastic equilibrium dialysis cell and a buffer that resembles the in vivo biochemical environment (Nelson JC
C Estrada et al.
The Biochemical journal, 326 ( Pt 2), 369-376 (1997-09-18)
Although it has been demonstrated that NO inhibits the proliferation of different cell types, the mechanisms of its anti-mitotic action are not well understood. In this work we have studied the possible interaction of NO with the epidermal growth factor
S Hugel et al.
British journal of pharmacology, 166(8), 2402-2416 (2012-03-29)
Artificial buffers such as HEPES are extensively used to control extracellular pH (pH(e) ) to investigate the effect of H(+) ions on GABA(A) receptor function. In neurones cultured from spinal cord dorsal horn (DH), dorsal root ganglia (DRG) and cerebellar
Jihye Park et al.
The Analyst, 137(19), 4411-4414 (2012-08-07)
We have developed a quickly responsive, and specific fluorescent assay for the detection of Au(III) on the basis of the formation of gold nanoparticles in the presence of HEPES, which cleave the C-I bond of I-BODIPY 1 to yield the
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