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G7295

Sigma-Aldrich

Anti-GM130 (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-GM130 (Golgi Matrix Protein of 130 kDa), Anti-Golgi Autoantigen, Anti-Golgi Matrix Protein GM 130

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~130 kDa

species reactivity

mouse, human, rat

technique(s)

immunoprecipitation (IP): 2-4 μg using Hela and 3T3 cell extracts.
indirect immunofluorescence: 0.2-0.4 μg/mL using rat NRK cells
western blot (chemiluminescent): 0.1-0.2 μg/mL using whole extract of rat NRK cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... GOLGA2(2801)
mouse ... Golga2(99412)
rat ... Golga2(64528)

General description

Golgi Matrix Protein of 130 kDa (GM130) is a peripheral cytoplasmic protein and is a member of the golgin family of Golgi autoantigens. GM130 is an extended rod-like protein containing coiled-coil domains and is tightly bound to Golgi membranes.

Immunogen

synthetic peptide corresponding to the C-terminal region of human GM130 with an N-terminal added lysine, conjugated to KLH. The corresponding sequence is identical in rat and mouse GM130.

Application

Anti-GM130 (C-terminal) antibody can be used in immunoblotting (approx. 130 kDa), indirect immunofluorescence (0.2-0.4 μg/mL using rat NRK cells), and immunoprecipitation (2-4 μg using Hela and 3T3 cell extracts). The antibody can also be used for chemiluminescent western blot (0.1-0.2 μg/mL using whole extract of rat NRK cells).
Anti-GM130 (C-terminal) antibody produced in rabbit has been used in:
  • immunoblotting
  • indirect immunofluorescence
  • immunoprecipitation
  • immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Golgi Matrix Protein of 130 kDa (GM130) is involved in maintaining the structural integrity of the Golgi apparatus and vesicular transport. GM130 also interacts with Golgi reassembly-stacking protein of 65 kDa (GRASP65) and participated in postmitotic reassembly and stacking of the Golgi cisternae. The protein along with p115 and GRASP65 might acts as a template in nucleating the formation of the Golgi apparatus. GM130 in complex with GRASP65 and other proteins interacts with activated Rab1, a protein known to regulate the transport of newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. This antibody can be used as a cis-Golgi network marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7,.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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The GM130 and GRASP65 Golgi proteins cycle through and define a subdomain of the intermediate compartment
Marra P, et al.
Nature Cell Biology, 3, 1101-1113 (2001)
Rab1 interaction with a GM130 effector complex regulates COPII vesicle cis-Golgi tethering
Moyer BD, et al.
Traffic, 2, 268-276 (2001)
Takanobu Nakazawa et al.
Nature communications, 7, 10594-10594 (2016-02-04)
Intracellular trafficking of receptor proteins is essential for neurons to detect various extracellular factors during the formation and refinement of neural circuits. However, the precise mechanisms underlying the trafficking of neurotrophin receptors to synapses remain elusive. Here, we demonstrate that
A novel Rab6-interacting domain defines a family of Golgi-targeted coiled-coil proteins
Barr FA, et al.
Current Biology, 9, 381-384 (1999)
dGRASP localization and function in the early exocytic pathway in Drosophila S2 cells
Kondylis V, et al.
Molecular Biology of the Cell, 16, 4061-4072 (2005)

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