A3275
Anti-Human IgM (μ-chain specific)−Alkaline Phosphatase antibody produced in goat
affinity isolated antibody, buffered aqueous glycerol solution
Synonym(s):
Goat Anti-Human IgM (μ-chain specific)−AP
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About This Item
Recommended Products
biological source
goat
Quality Level
conjugate
alkaline phosphatase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous glycerol solution
species reactivity
human
technique(s)
direct ELISA: 1:7,000-1:21,000
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
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General description
IgM antibody is produced by B cells and has pentamer structure which helps the antibody in polyreactivity and can also remove apoptotic cells. Anti-human IgM (μ-chain specific) -alkaline phosphatase antibody can be used in solid phase ELISA for determination of anti-sp75 antibodies. Goat anti human IgM-alkaline phosphatase antibody reacts specifically with human IgM.
Immunogen
Human IgM.
Application
IgM is a glycoprotein with 5 n-linked glycosylation sites on the heavy chain. An ELISA assay was performed to identify glycosylated forms of IgM that bind to lectin. Alkaline phosphatase conjugated goat anti-human IgM was used as the secondary at 1:2000 and developed using p-nitrophenyl substrate (Sigma).
It may be used for immunoblotting.
Physical form
Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 15 mM sodium azide and 50% glycerol
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Immunoglobulin M Reactivity Towards the Immunologicdly Active Region sp75 of the Core Protein of Hepatitis C Virus (HCV) in Chronic HCV Infection.
U.B. Hellstrorn, S.P.E. Sylvan
Journal of Medical Virology, 39(4), 39325-39332 (1993)
Mandar Patgaonkar et al.
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Tzer Chyn Lim et al.
Current biology : CB, 28(6), 955-962 (2018-03-06)
The position of the division site dictates the size and fate of daughter cells in many organisms. In animal cells, division-site placement involves overlapping mechanisms, including signaling from the central spindle microtubules, astral microtubules, and spindle poles and through polar
Songwang Hou et al.
Journal of immunological methods, 440, 27-34 (2016-10-30)
The presence of circulating autoantibodies against phosphatidylethanolamine (PE) has been shown to be positively associated with symptoms of antiphospholipid syndromes (APS). However, the current ELISA-based tests for antiphosphatidylethanolamine (aPE) antibodies remain inconsistent and controversial. The term PE refers to a
Henrik Chart et al.
FEMS immunology and medical microbiology, 47(3), 391-397 (2006-07-29)
The techniques of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting were evaluated for the serodiagnosis of human infections with Yersinia enterocolitica and Yersinia pseudotuberculosis. Lipopolysaccharide (LPS) was prepared from strains comprising four serogroups of Y. enterocolitica and five
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