Preparation of homogeneous samples of double-labelled protein suitable for single-molecule FRET measurements.: This study explores the use of Atto 647N maleimide for the preparation of homogeneously double-labelled protein samples, facilitating precise single-molecule FRET measurements to analyze protein dynamics and interactions (Lerner et al., 2013).
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The Journal of endocrinology, 206(1), 27-36 (2010-04-16)
Adipose tissue is a highly active endocrine organ secreting a variety of signaling molecules called adipokines. Leptin and resistin are two adipokines critically involved in metabolic homeostasis. Nevertheless, the secretory pathways of these adipokines and their interplays are poorly elucidated.
Single-molecule STED microscopy with photostable organic fluorophores.
Robert Kasper et al.
Small (Weinheim an der Bergstrasse, Germany), 6(13), 1379-1384 (2010-06-04)
Accurate tumor localization is essential for gastrointestinal surgery, especially in cases of early cancer. This study was designed to develop a novel fluorescent clip for rapid and exact visualization of tumor sites. A transparent polymer matrix containing highly bright fluorochromes
Cholesterol-mediated lipid interactions are thought to have a functional role in many membrane-associated processes such as signalling events. Although several experiments indicate their existence, lipid nanodomains ('rafts') remain controversial owing to the lack of suitable detection techniques in living cells.
A novel lateral flow immunoassay (LFIA) signal amplification strategy for the detection of Cry1Ab based on amplification via a polylysine (PL) chain and biotin-streptavidin system (BSAS) is described. In this system, multiple fluorescence dyes (FL) were directly coated on the
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