10102105001
Roche
Adenosine Deaminase (ADA)
from calf intestine
Synonym(s):
ADA
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
biological source
bovine (calf) intestine
Quality Level
form
solution
specific activity
~200 units/mg protein (At 25 °C with adenosine as the substrate.)
mol wt
230-440 kDa (high)
30-47 kDa (low )
packaging
pkg of 2 mL (10 mg)
manufacturer/tradename
Roche
optimum pH
7.0-7.4
shipped in
wet ice
storage temp.
2-8°C
Related Categories
General description
Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum. It is also called Adenosine aminohydrolase. It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.
Adenosine deaminase (ADA) is a zinc metalloenzyme.
Application
Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.
Adenosine deaminase has been used to pre-treat primary Kupffer cells.
Biochem/physiol Actions
Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs. It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti. ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.
Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.
Quality
Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each
Physical form
Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6
Preparation Note
Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Adenosine deaminase deficiency: a review
Flinn AM and Gennery AR
Orphanet Journal of Rare Diseases, 13(1), 65-65 (2018)
Pdx-1 is required for activation in vivo from a duodenum-specific enhancer
Dusing MR, et al.
The Journal of biological chemistry, 276(17), 14434-14442 (2001)
Purification and characterization of intestinal adenosine deaminase from mice
Singh L and Sharma R
Molecular and Cellular Biochemistry, 204(1-2), 127-134 (2000)
Development of adenosine deaminase-specific IgY antibodies: Diagnostic and inhibitory application
Lupicka-Slowik A, et al.
Applied Biochemistry and Biotechnology, 184(4), 1358-1374 (2018)
Mi-Jeong Lee et al.
Methods in enzymology, 538, 49-65 (2014-02-18)
Obesity is reaching epidemic proportions so there is growing interest in the mechanisms that regulates adipose tissue development and function. Although murine adipose cell lines are useful for many mechanistic studies, primary human adipose stromal cells (ASCs), which can be
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service