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PTHT24H48

Millipore

Millicell® 24 well hanging cell culture inserts

pore size 0.4 μm, translucent PET membrane, diam. 6.5 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

cell culture inserts, membrane insert, plate inserts

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About This Item

UNSPSC Code:
41122107
NACRES:
NB.13

material

polystyrene housing
translucent PET membrane

Quality Level

sterility

γ-irradiated
sterile

feature

100x106 pores/cm2

packaging

pack of 48 ea (individually blister packaged)

parameter

max. temp. at 37 °C

technique(s)

cell attachment: suitable
cell differentiation: suitable
drug transporter assay: suitable
permeability assay: suitable

H

15.5 mm

diam.

6.5 mm

insert maximum volume

0.4 mL

insert working volume

0.2 mL

membrane thickness

9-16 μm

size

24 wells

surface area

0.3 cm2

color

translucent membrane

pore size

0.4 μm

binding type

Tissue Culture (TC)-treated surface

General description

Millicell® Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has an high pore density translucent 0.4 μm pore size membrane and fits in most 24-well plastic tissue culture plates.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • In Vitro Toxicology
  • Microbial Attachment
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport/Permeability

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Discover tips for choosing the best plate to use with Millicell® hanging inserts for your applications, including barrier formation and cell imaging.

Discover tips for choosing the best plate to use with Millicell® hanging inserts for your applications, including barrier formation and cell imaging.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

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Protocols

3D cell culture protocol for generating epidermal human skin tissue using primary human keratinocytes, dermal fibroblasts, and collagen-coated transwell inserts.

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