MAB5412
Anti-Indoleamine 2,3-dioxygenase Antibody, clone 10.1
clone 10.1, Chemicon®, from mouse
Synonym(s):
Indoleamine-pyrrole 2,3-dioxygenase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
10.1, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG3
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... IDO1(3620)
General description
Indoleamine 2,3-dioxygenase (IDO) is an enzyme that is responsible for converting tryptophan to kynurenines. IDO is expressed by a wide variety of tissues and IDO can be upregulated by interferon gamma. IDO modulates levels of the amino acid tryptophan, which is vital for cell growth, but is also involved in the suppression of the immune response. IDO may be involved in the suppression of the immune response to tumours and blocking the IDO pathway may be a potential target for immunotherapy.
Specificity
Reacts with Indolamine 2,3-dioxygenase (IDO). Note IDO is usually expressed in response to various stimuli. A good positive control is HeLa cells stimulated with IFN-gamma, {http://www.upstate.com/browse/productdetail.q.ProductID.e.05-840#}.
Immunogen
Amino acids 78-184 of human IDO fused to GST.
Application
Anti-Indoleamine 2, 3-dioxygenase Antibody, clone 10.1 is an antibody against Indoleamine 2 for use in IH & WB.
Immunohistochemistry:
A previous lot of this antibody worked on lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended.
Western blot:
Recognizes a 42-45kDa band in IFN-gamma treated human HeLa cell lines. Mouse IDO runs slightly smaller in blots than human IDO.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody worked on lightly fixed tissues; Triton X-100 treatment only in the block, not with primary antibody. High temperature antigen retrieval is recommended.
Western blot:
Recognizes a 42-45kDa band in IFN-gamma treated human HeLa cell lines. Mouse IDO runs slightly smaller in blots than human IDO.
Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms
Inflammation & Autoimmune Mechanisms
Quality
Routinely evaluated by Western Blot on untreated and IFNgamma treated HeLa lysates.
Western blot:
1:500 dilution of this lot detected IDO on 10 μg of untreated and IFN gamma treated HeLa lysates.
Western blot:
1:500 dilution of this lot detected IDO on 10 μg of untreated and IFN gamma treated HeLa lysates.
Target description
42-45kDa
Linkage
Replaces: AB5968
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG3 in buffer containing 0.02 M phosphate, 0.25 M NaCl, 0.1% NaN3, pH 7.6.
Storage and Stability
Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.
Analysis Note
Control
IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
IFN-gamma stimulated human peripheral blood lymphocytes, HeLa lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Davide Matino et al.
The Journal of clinical investigation, 125(10), 3766-3781 (2015-10-02)
The development of inhibitory antibodies to factor VIII (FVIII) is a major obstacle in using this clotting factor to treat individuals with hemophilia A. Patients with a congenital absence of FVIII do not develop central tolerance to FVIII, and therefore
Maria Grazia Iachininoto et al.
Molecules (Basel, Switzerland), 18(9), 10132-10145 (2013-08-27)
Indoleamine 2,3-dioxygenase 1 (IDO1) metabolizes L-tryptophan to kynurenines (KYN), inducing T-cell suppression either directly or by altering antigen-presenting-cell function. Cyclooxygenase (COX)-2, the rate-limiting enzyme in the synthesis of prostaglandins, is over-expressed by several tumours. We aimed at determining whether COX-2
Antonella De Luca et al.
PLoS pathogens, 9(7), e1003486-e1003486 (2013-07-16)
The ability to tolerate Candida albicans, a human commensal of the gastrointestinal tract and vagina, implicates that host defense mechanisms of resistance and tolerance cooperate to limit fungal burden and inflammation at the different body sites. We evaluated resistance and
Sung Hoon Sim et al.
Cancer science, 103(2), 155-160 (2011-11-24)
There is no specific marker to evaluate the immuno-suppressive status of cancer patients. Several markers, such as CD124, latency-associated peptide (LAP), arginase I, indole-amine-2,3-dioxygenase (IDO) and inducible nitric oxide synthase (iNOS), are known to be associated with immune suppression. However
Hatem Soliman et al.
Cancer immunology, immunotherapy : CII, 62(5), 829-837 (2013-01-25)
The immunosuppressive enzyme, indoleamine 2,3 dioxygenase (IDO), is overexpressed in many different tumor types including breast cancer. IDO inhibitors synergize with chemotherapy in breast cancer murine models. Characterizing IDO expression in breast cancer could define which patients receive IDO inhibitors.
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