12024AST

Astec^® CHIROBIOTIC^® T Chiral (5 μm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• Synonym(s): CHIROBIOTIC T Column
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: Astec^® CHIROBIOTIC^® T Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L63
• description: HPLC column
• product line: Astec^®
• packaging: pkg of 1 ea
• manufacturer/tradename: Astec^®
• parameter: 0-45 °C temperature; 241 bar pressure (3500 psi)
• technique(s): HPLC: suitable; LC/MS: suitable
• L × I.D.: 25 cm × 4.6 mm
• matrix: High-purity silica gel particle platform; fully porous particle
• matrix active group: teicoplanin glycopeptide phase
• particle size: 5 μm
• pore size: 100 Å
• operating pH range: 3.8-6.8
• separation technique: chiral

Description

General description

CHIROBIOTIC^® T and T2 have teicoplanin as the chiral selector. They offer unique selectivity for a number of classes of molecules, specifically underivatized α, β, γ and cyclic amino acids, N-derivatized amino acids, hydroxy-carboxylic acids, acidic compounds including carboxylic acids and phenols, small peptides, neutral aromatic analytes and cyclic aromatic and aliphatic amines. Separations normally obtained on a chiral crown ether or ligand exchange-type CSPs are also possible on the CHIROBIOTIC^® T and T2, but in much simpler mobile phases, like water-alcohol. In addition, all of the known beta-blockers (amino alcohols), and dihydrocoumarins have been resolved. CHIROBIOTIC^® T and T2 differ in their bonding chemistry and the pore size of the support particle, giving them different selectivity and preparative capacity.

• Bonded phase: Teicoplanin
• Operating pH range: 3.8 - 6.8
• Particle diameter: 5, 10 or 16 μm
• Pore size: 100 Å (CHIROBIOTIC^® T) or 200 Å (CHIROBIOTIC^® T2)

CHIROBIOTIC FAQs
CHIROBIOTIC Reference Bibliography
Chiral Product Literature

Application

• Chiral liquid chromatography-mass spectrometry (LC-MS/MS) method development for the detection of salbutamol in urine samples.: This study utilizes the Astec CHIROBIOTIC T Chiral HPLC Column to develop an LC-MS/MS method for detecting and quantifying salbutamol enantiomers in urine samples. This application demonstrates the column′s utility in biomedical and clinical research, particularly in the analysis of chiral drugs in biological matrices (Chan et al., 2016).


• High-performance liquid chromatographic enantioseparation of 2-aminomono- and dihydroxycyclopentanecarboxylic and 2-aminodihydroxycyclohexanecarboxylic acids on macrocyclic glycopeptide-based phases.: This research focuses on the enantioseparation of various amino acids using the Astec CHIROBIOTIC T Chiral HPLC Column, highlighting its effectiveness in separating complex chiral molecules. The study underscores the column′s application in the enantioselective analysis of amino acids and related compounds in chemical and pharmaceutical research (Berkecz et al., 2009).

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Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable