T4661

Trizma^® base

≥99.9% (titration), crystalline

• Synonym(s): 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
• Linear Formula: NH₂C(CH₂OH)₃
• CAS Number: 77-86-1
• Molecular Weight: 121.14
• Beilstein: 741883
• EC Number: 201-064-4
• MDL number: MFCD00004679
• UNSPSC Code: 12352104
• PubChem Substance ID: 24900061
• NACRES: NA.25

Properties

• description: aminopeptidase substrate
• Quality Level: 400
• Assay: ≥99.9% (titration)
• form: crystalline
• loss: ≤5% (loss on drying, 110 °C)
• color: white
• pH: 10.0-11.5
• useful pH range: 7-9
• pKa (25 °C): 8.1
• bp: 219-220 °C/10 mmHg (lit.)
• mp: 167-172 °C (lit.)
• solubility: water: soluble (400 g plus 600 mL of water)
• λ: (290nm < 0.05); in 5 mM Tris; DNA (40% (w/w))
• application(s): diagnostic assay manufacturing
• SMILES string: NC(CO)(CO)CO
• InChI: 1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
• InChI key: LENZDBCJOHFCAS-UHFFFAOYSA-N

Description

General description

Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]

Application

Tris base has been used:

• As a component of lysis buffer for cell disruption[4][5]
• In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]
• In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]
• In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]

Packaging

Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.

Analysis Note

Not pyrogen tested. Certificates of analysis are available with lot specific information.

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves