Fifteen laminin trimers have been identified. We do not determine what type of laminin this is. Typically laminin from placenta is considered laminin-2/4 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane is laminin-1.
L6274
Laminin from human placenta
liquid, BioReagent, suitable for cell culture
Laminin from human placenta
Synonym(s):
Cell culture grade laminin, Human Laminin
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|
About This Item
Form:
liquid
Biological source:
human placenta
Pricing and availability is not currently available.
biological source
human placenta
Quality Segment
product line
BioReagent
form
liquid
concentration
0.5 mg/mL in Tris-buffered saline
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑2 μg/cm2
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... LAMB1(3912)
Application
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Biochem/physiol Actions
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.
Preparation Note
This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.
Other Notes
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.
Disclaimer
It is recommended to store this product at -70°C, where it will remain stable for two years.
1 of 1
This Item | |||
|---|---|---|---|
| biological source human placenta | biological source human fibroblasts | biological source mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane) | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| concentration 0.5 mg/mL in Tris-buffered saline | concentration - | concentration 1-2 mg/mL in Tris-buffered saline | concentration - |
| form liquid | form liquid | form aqueous solution | form solution |
| Gene Information human ... LAMB1(3912) | Gene Information human ... LAMB1(3912) | Gene Information mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782) | Gene Information mouse ... Lamb2(16779) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Is Product L6274, Laminin from human placenta, laminin 1 or 2?
1 answer-
Helpful?
-
-
How do you prepare Product L6274, Laminin from human placenta?
1 answer-
To prepare a laminin solution, we recommend that the L6274 be thawed at 2-8°C to avoid the formation of a gel. This can be further diluted in a balanced salt solution. If the product has been thawed at room temperature and ends up as a gel, it cannot be reactivated for use.
Helpful?
-
-
How long can cover slips coated with Product L6274, Laminin from human placenta, be stored?
1 answer-
Laminin coatings, such as laminin coated cover slips last about one month at 2-8°C. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-