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Ethylenediaminetetraacetic acid solution

Name: Ethylenediaminetetraacetic acid solution
Brand: SIGMA

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

EDTA solution

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About This Item

Empirical Formula (Hill Notation):

C₁₀H₁₆N₂O₈

CAS Number:

60-00-4

Molecular Weight:

292.24

NACRES:

NA.75

PubChem Substance ID:

24894664

eCl@ss:

39030907

UNSPSC Code:

12352204

MDL number:

MFCD00003541

Beilstein/REAXYS Number:

1716295

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Properties

sterility

sterile-filtered

Quality Segment

200

product line

BioReagent

form

solution

concentration

0.02% in DPBS (0.5 mM)

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.1-7.7

shipped in

wet ice

storage temp.

2-8°C

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

Description

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

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This Item	ED	E9884	RDD017
Sigma-Aldrich E8008 Ethylenediaminetetraacetic acid solution	Sigma-Aldrich ED Ethylenediaminetetraacetic acid	Sigma-Aldrich E9884 Ethylenediaminetetraacetic acid	Sigma-Aldrich RDD017 Ethylenediaminetetraacetic acid
description 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture	description purified grade, ≥98.5%, powder	description ACS reagent, 99.4-100.6%, powder	description anhydrous, free-flowing, powder, Redi-Dri^™, ACS reagent, 99.4-100.6%
technique(s) cell culture \| mammalian: suitable	technique(s) -	technique(s) -	technique(s) cell culture \| mammalian: suitable
product line BioReagent	product line -	product line -	product line Redi-Dri^™
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
form solution	form powder	form powder	form powder
impurities endotoxin, tested	impurities -	impurities ≤0.005% insoluble matter, ≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)	impurities ≤0.005% Insol. Dil. NH₄OH, ≤0.1% Nitrilotriacetic acid [[HOCOCH₂₃N]
storage temp. 2-8°C	storage temp. -	storage temp. -	storage temp. -

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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